Modulation of Glucose Homeostasis in Rats Treated with Bacterial Lipopolysaccharide: Role of L-Arginine Counter-Strategy | ||
| SVU-International Journal of Veterinary Sciences | ||
| Volume 5, Issue 4 - Serial Number 14, December 2022, Pages 138-152 PDF (1.08 M) | ||
| Document Type: Research article | ||
| DOI: 10.21608/svu.2022.153308.1218 | ||
| Authors | ||
| Azza A. Abd Elkareem Abd Elkareem1; Zeinab Al-Amgad2; Hassan Ahmed* 1 | ||
| 1Physiology department, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt | ||
| 2General Authority for Veterinary Services, Ph.D in Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt | ||
| Abstract | ||
| The purpose of this study was to assess the effect of L-arginine on pancreas function in rats treated with LPS. Therefore, rats were weighed, randomly divided into 4 groups, and intraperitoneally injected as follows: Control group (saline), LPS group, L-Arg group (for 7 days), and LPS+L-Arg group (for 7 days then once injected with LPS). Serum IL-6, glucose, and insulin levels were assessed at 6, 12, 24, and 72 hours after last injection. Histological examination of the pancreas tissue was performed. Serum IL-6 elevated significantly 6 and 12 hours after LPS injection and retrieved at 24 and 72 hours. Association of LPS and L-Arg potentiate IL-6 production at 6 and 12 hours higher than that in L-Arg-treated group. Serum glucose was declined in single LPS-treated rats 6 and 12 hours and recovered at 24 and 72 hours after LPS injection. On contrary, Serum glucose were elevated 6 and 12 hours and declined near to control level 24 and 72 h after L-Arg injection. However, insulin levels were slightly elevated 12, 24 and 72 hours after LPS injection and 12 hours after LPS+L-Arg injection the rescued around the normal values at 24 and 72 hours. On contrary, serum insulin level decrease at 6 hours after L-Arg injection then recovered to control level. Histologically, rats treated with LPS showed necrosis, vacuolation in islets of Langerhans with infiltration of inflammatory cells. Pre-treatment of L-Arg prior LPS attenuated the alteration in pancreas tissue architecture however, rats treated with L-Arg showed normal pancreas tissue. | ||
| Keywords | ||
| Lipopolysaccharides; L-Arginine; Nitric Oxide; Insulin; Pancreas | ||
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