Detection of cnf1 and cnf2 Genes in Clinical Isolates of E.coli
khalaf, Z., Flayyih, M. (2022). Detection of cnf1 and cnf2 Genes in Clinical Isolates of E.coli. EKB Journal Management System, 89(2), 8088-8094. doi: 10.21608/ejhm.2022.277598
zainab zamel khalaf; May Talib Flayyih. "Detection of cnf1 and cnf2 Genes in Clinical Isolates of E.coli". EKB Journal Management System, 89, 2, 2022, 8088-8094. doi: 10.21608/ejhm.2022.277598
khalaf, Z., Flayyih, M. (2022). 'Detection of cnf1 and cnf2 Genes in Clinical Isolates of E.coli', EKB Journal Management System, 89(2), pp. 8088-8094. doi: 10.21608/ejhm.2022.277598
khalaf, Z., Flayyih, M. Detection of cnf1 and cnf2 Genes in Clinical Isolates of E.coli. EKB Journal Management System, 2022; 89(2): 8088-8094. doi: 10.21608/ejhm.2022.277598

Detection of cnf1 and cnf2 Genes in Clinical Isolates of E.coli

The Egyptian Journal of Hospital Medicine
Article 299, Volume 89, Issue 2, October 2022, Page 8088-8094  XML PDF (712.28 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2022.277598
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Authors
zainab zamel khalaf email ; May Talib Flayyih
Department of biology/College of science/University of Baghdad
Abstract
Background: CNF are single-chain proteins that are created by some uropathogenic Escherichia coli.There are three types of CNFs detected in E.coli, but CNF1 represent more frequently.
Objective: The purposes of this study were to determine the prevalence of cnf1 and cnf2 genes in  E. coli isolates by using specific primers.
Materials and Methods: The sensitivity test was evaluated by using different types of antibiotics in the current study, and the cnf1 and cnf2 genes were detected by PCR in all bacterial isolates.
Results: The antibiotics sensitivity test show variable degrees of sensitivity and resistance. The high percentage of sensitivity was achieved against amikacin at a percentage of 86% and ciprofloxacin, ceftazidime, gentamicin, and tobramycin at a percentage of 80%, whereas trimethoprim and aztreonam at 64%and 60% respectively. In this study, most isolates were resistant to Amoxicillin (92%);  while showing different degrees of resistance against other types of antibiotics ranging from Tetracycline (62%) to amikacin 4%.  The frequency of MDR bacteria was about 64 % (32 isolates), 30 from urine, and 2 others from stool. The results revealed that the cnf1 gene was found in Five bacterial isolates (10%), whereas the other 45 (90%) isolates don’t have this gene, while cnf2 wasn’t found in any one of the bacterial isolates.
Conclusion: E.coli was recorded as multidrug resistance (MDR) and the cnf1 gene was found only in uropathogenic isolates.
 
Keywords
Cytotoxic necrotizing factor (CNF); E. coli; antibiotics; genotoxins
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