Neutrophil Gelatinase-associated Lipocalin-2 and Macrophage antigen -1 in Cirrhotic Patients Infected with Carbapenem Resistant Enterobacteriaceae
khalil, F., Mandour, S., Bedira, I., Elkhadry, S., Ibrahim, A., Abdelmageed, N., El -shemy, E., El-refai, H. (2023). Neutrophil Gelatinase-associated Lipocalin-2 and Macrophage antigen -1 in Cirrhotic Patients Infected with Carbapenem Resistant Enterobacteriaceae. EKB Journal Management System, 32(1), 1-11. doi: 10.21608/ejmm.2023.277762
fatma o khalil; Sally Saad Mandour Saad Mandour; Isis Samy Bedira; Sally Elkhadry; Amr Ragab Ibrahim; Neamat Abdelmageed; Eman El -shemy; Hala Ahmed El-refai. "Neutrophil Gelatinase-associated Lipocalin-2 and Macrophage antigen -1 in Cirrhotic Patients Infected with Carbapenem Resistant Enterobacteriaceae". EKB Journal Management System, 32, 1, 2023, 1-11. doi: 10.21608/ejmm.2023.277762
khalil, F., Mandour, S., Bedira, I., Elkhadry, S., Ibrahim, A., Abdelmageed, N., El -shemy, E., El-refai, H. (2023). 'Neutrophil Gelatinase-associated Lipocalin-2 and Macrophage antigen -1 in Cirrhotic Patients Infected with Carbapenem Resistant Enterobacteriaceae', EKB Journal Management System, 32(1), pp. 1-11. doi: 10.21608/ejmm.2023.277762
khalil, F., Mandour, S., Bedira, I., Elkhadry, S., Ibrahim, A., Abdelmageed, N., El -shemy, E., El-refai, H. Neutrophil Gelatinase-associated Lipocalin-2 and Macrophage antigen -1 in Cirrhotic Patients Infected with Carbapenem Resistant Enterobacteriaceae. EKB Journal Management System, 2023; 32(1): 1-11. doi: 10.21608/ejmm.2023.277762

Neutrophil Gelatinase-associated Lipocalin-2 and Macrophage antigen -1 in Cirrhotic Patients Infected with Carbapenem Resistant Enterobacteriaceae

Egyptian Journal of Medical Microbiology
Volume 32, Issue 1, January 2023, Pages 1-11    PDF (549.43 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2023.277762
Authors
fatma o khalil* 1; Sally Saad Mandour Saad Mandour2; Isis Samy Bedira2; Sally Elkhadry3; Amr Ragab Ibrahim4; Neamat Abdelmageed5; Eman El -shemy5; Hala Ahmed El-refai6
1Microbiology nd Immunology, National Liver Institute, Menoufia University, Shebin El-kom, Egypt
2Clinical Pathology Department, National Liver Institute Menoufia University
3Epidemiology and Preventive Medicine, National Liver Institute Menoufia University
4Hepatology and Gastroenterology Department, National Liver Institute Menoufia University
55Department of Hepatogastroentrology & Infectious Diseases, Faculty of Medicine for girls, Al-Azhar University
6Microbiology nd Immunology, National Liver Institute, Menoufia University, Shebin El-kom
Abstract
Background: Early detection of bacterial infection in patients with cirrhosis remains a hope for improved prognosis to decrease the morbidity and mortality rate. Objective: To study the diagnostic importance of Neutrophil gelatinase-associated lipocalin-2 and macrophage antigen -1 for early detection of infection in cirrhotic patients and their relationship with carbapenem resistant bacteria. Methodology: 150 patients were divided into group I: cirrhotic patients complicated with infection subdivided into three subgroups; A: fifty-five patients with spontaneous bacterial peritonitis (SBP), Subgroup B: fifteen patients with culture-negative neutrocytic ascites (CNNA) and subgroup C: thirty patients with urinary tract infection. Group II: fifty cirrhotic patients without infections. NGAL-2 estimation was done by ELISA, neutrophil MAC-1 by flowcytometry and Carbapenem resistance genes; OXA-48 and KPC-3 were detected by RFLP-PCR analysis Results: Significant increase of ascitic fluid neutrophils and WBCs count in SBP subgroup. Mean fluorescence intensity of Mac-1 and NGAL-2 concentrations were significantly higher in cirrhotic infected than uninfected patients. The OXA-48 and KPC-3 Carbapenem resistant gram-negative bacteria were prevalent among cirrhotic infected patients. NGAL-2 can detect early infection in cirrhotic patients followed by Mac-1. Conclusion: NGAL-2 and Mac-1 could be used for early detection of bacterial infection in cirrhotic patients with high specificity and sensitivity.
Keywords
Mac-1; NGAI-2; SBP; PCR; flow cytometer; Cirrhosis
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