SARS-COV-2 antibody testing Versus Nasopharyngeal and Oropharyngeal swab Sample Polymerase Chain Reaction for Diagnosis of Coronavirus Disease 2019 (COVID-19) in Egyptian Patient. A Comparative study
khalil, F., Abd ElAziz, A., Abedelrahman, M., Abedelrahman, A., Abed El motaleb, T. (2023). SARS-COV-2 antibody testing Versus Nasopharyngeal and Oropharyngeal swab Sample Polymerase Chain Reaction for Diagnosis of Coronavirus Disease 2019 (COVID-19) in Egyptian Patient. A Comparative study. EKB Journal Management System, 32(1), 87-95. doi: 10.21608/ejmm.2023.277784
fatma o khalil; Azza Abd ElAziz; mohamed Abedelrahman; Ahmed Abedelrahman; Tawfik Mohamed Abed El motaleb. "SARS-COV-2 antibody testing Versus Nasopharyngeal and Oropharyngeal swab Sample Polymerase Chain Reaction for Diagnosis of Coronavirus Disease 2019 (COVID-19) in Egyptian Patient. A Comparative study". EKB Journal Management System, 32, 1, 2023, 87-95. doi: 10.21608/ejmm.2023.277784
khalil, F., Abd ElAziz, A., Abedelrahman, M., Abedelrahman, A., Abed El motaleb, T. (2023). 'SARS-COV-2 antibody testing Versus Nasopharyngeal and Oropharyngeal swab Sample Polymerase Chain Reaction for Diagnosis of Coronavirus Disease 2019 (COVID-19) in Egyptian Patient. A Comparative study', EKB Journal Management System, 32(1), pp. 87-95. doi: 10.21608/ejmm.2023.277784
khalil, F., Abd ElAziz, A., Abedelrahman, M., Abedelrahman, A., Abed El motaleb, T. SARS-COV-2 antibody testing Versus Nasopharyngeal and Oropharyngeal swab Sample Polymerase Chain Reaction for Diagnosis of Coronavirus Disease 2019 (COVID-19) in Egyptian Patient. A Comparative study. EKB Journal Management System, 2023; 32(1): 87-95. doi: 10.21608/ejmm.2023.277784

SARS-COV-2 antibody testing Versus Nasopharyngeal and Oropharyngeal swab Sample Polymerase Chain Reaction for Diagnosis of Coronavirus Disease 2019 (COVID-19) in Egyptian Patient. A Comparative study

Egyptian Journal of Medical Microbiology
Volume 32, Issue 1, January 2023, Page 87-95  XML PDF (342.22 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2023.277784
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Authors
fatma o khalil email orcid 1; Azza Abd ElAziz2; mohamed Abedelrahman3; Ahmed Abedelrahman3; Tawfik Mohamed Abed El motaleb4
1Microbiology nd Immunology, National Liver Institute, Menoufia University, Shebin El-kom, Egypt
2Departement of Clinical Microbiology and Immunology, National Liver Institute, Menoufia University Menoufia, Egypt
3Medical Military Academy, Alexandria, Egypt
4Professor of Medical Microbiology and Immunology, Clinical Microbiology and Immunology department, National Liver Institute, Menoufia University, Shebin El-kom 32511, Egypt,
Abstract
Background: SARS-CoV-2 identification via Real-time polymerase chain reaction (RT-PCR) testing considered the current standard for the diagnosis of infection, but its utilization for large-scale screening is limited. Serological immunoglobulin M (IgM)/IgG testing has been proposed as a useful tool for detecting SARS-CoV-2 exposure, Objectives; to assess the diagnostic value of SARS-COV-2 IgM and IgG in early diagnosis of patient with COVID-19 versus viral RNA detection by PCR and to Investigate the sensitivity of nasopharyngeal swabs versus oropharyngeal samples for early detection of SARS-COV-2. Methodology:  This study was carried on 200 COVID-19 PCR positive cases divided into two groups as (Group I). In addition to 200 apparently healthy individuals (Negative PCR) as a control group (Group II) obtained From Ministry of Health Hospitals after getting approval. All patients were subjected to: clinical examination, lab investigation including; SARS-COV-2 IgM and IgG testing, SARS-COV-2 PCR test from nasopharyngeal and Oropharyngeal swabs samples, Results; Regarding IgM after 10 days, It was positive in 77 (38.5 %) of patients with Positive PCR while no one was positive in patients with negative PCR with sensitivity of 38.5 % , Specificity 100.0 %, and accuracy 69.25 %, Nasopharyngeal Swabs (NPS) had significantly higher SARS-CoV-2 detection rate, sensitivity, and viral load than Oropharyngeal Swabs. NPS could reduce droplets production during swabs. Conclusion: NPS should be recommended for diagnosing COVID-19 and monitoring SARS-CoV-2 load. Also, our study analyzed the clinical performance of the rapid serological test and confirmed the test’s limited applicability for the diagnosis of SARS-CoV-2 infection.
Keywords
COVID-19; Oropharyngeal and Nasopharyngeal swabs; PCR; Serological tests
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