Characterization of β-Lactamase Genes among Multidrug- and Extensively Drug-Resistant Acinetobacter and Enterobacteriaceae Species Causing Infections in Critically Ill Patient
Khalifa, R., Aljahdali, H., Alghamdi, H., Ismail, B., Joharjy, H., Eibani, K. (2023). Characterization of β-Lactamase Genes among Multidrug- and Extensively Drug-Resistant Acinetobacter and Enterobacteriaceae Species Causing Infections in Critically Ill Patient. EKB Journal Management System, 32(1), 113-123. doi: 10.21608/ejmm.2023.277787
Reham Ali Khalifa; Hani Aljahdali; Huda Alghamdi; Bothayna Ismail; Husam Joharjy; Khalid Eibani. "Characterization of β-Lactamase Genes among Multidrug- and Extensively Drug-Resistant Acinetobacter and Enterobacteriaceae Species Causing Infections in Critically Ill Patient". EKB Journal Management System, 32, 1, 2023, 113-123. doi: 10.21608/ejmm.2023.277787
Khalifa, R., Aljahdali, H., Alghamdi, H., Ismail, B., Joharjy, H., Eibani, K. (2023). 'Characterization of β-Lactamase Genes among Multidrug- and Extensively Drug-Resistant Acinetobacter and Enterobacteriaceae Species Causing Infections in Critically Ill Patient', EKB Journal Management System, 32(1), pp. 113-123. doi: 10.21608/ejmm.2023.277787
Khalifa, R., Aljahdali, H., Alghamdi, H., Ismail, B., Joharjy, H., Eibani, K. Characterization of β-Lactamase Genes among Multidrug- and Extensively Drug-Resistant Acinetobacter and Enterobacteriaceae Species Causing Infections in Critically Ill Patient. EKB Journal Management System, 2023; 32(1): 113-123. doi: 10.21608/ejmm.2023.277787

Characterization of β-Lactamase Genes among Multidrug- and Extensively Drug-Resistant Acinetobacter and Enterobacteriaceae Species Causing Infections in Critically Ill Patient

Egyptian Journal of Medical Microbiology
Volume 32, Issue 1, January 2023, Pages 113-123    PDF (681.96 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2023.277787
Authors
Reham Ali Khalifa* 1; Hani Aljahdali2; Huda Alghamdi3; Bothayna Ismail4; Husam Joharjy5; Khalid Eibani6
1Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University
2Medical Department, King Abdulaziz Hospital, Jeddah, Saudia Arabia
3Laboratory Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia
4Clinical Pathology Department, Faculty of Medicine, Al Azhar University, Egypt.
5Infection Control Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia
6Surgery Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia
Abstract
Background: Multidrug resistant infection is the leading cause of ICU morbidity and mortality. Gram-negative bacilli are the most prevalent pathogens with Acinetobacter being the most resistant. Objectives: Phenotypic and genotypic characterization of multidrug- and extensively drug-resistant Acinetobacter and Enterobacteriaceae species causing infections in critically ill patients with detection of targeted β-lactamase genes and its impact on patients' morbidity and mortality. Methodology: Antibiotic susceptibility testing was done. Genes for class A and class B β-lactamases were detected by multiplex polymerase chain reaction (PCR) and 5 carbapenemases genes were detected by real time PCR using GeneXpert Carba-R system. Results: 16.6 % of A. baumannii were MDR, 50% were XDR, 50% of K. pneumoniae were XDR and 100% of P. stuartii were XDR. Detected genes included blaSHV, blaCTX-M, blaGIM-1, blaSIM-1, blaOXA-48 and blaNDM-1. Conclusion: MDR/XDR infections had a significant impact on patients’ mortality. Molecular epidemiology is crucial to guide infection control committees about preventive measures that can contain MDR/XDR infections and prevent their detrimental effect on patients’ outcome.
Keywords
Antimicrobial; Resistance; Gram-negative; Carbapenemase; Carbapenems
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