Moringa Oleifera Extract Could Ameliorate Aging-Related Structural and Functional Hepatic Changes by Regulating Autophagy Signaling Pathway | ||||
| Egyptian Journal of Histology | ||||
| Article 4, Volume 47, Issue 4, December 2024, Page 1297-1306 PDF (817.29 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2023.172750.1802 | ||||
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| Authors | ||||
Heba Osama Mohammed   1; rania saad ramadan2; Amal Fawzy3; Aliaa Talaat 3; Eman Alaa4; Maha M. Ahmed Abdul Rahman 5
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| 1human anatomy and embryology,faculty of medicine,Zagazig University | ||||
| 2Human anatomy and embryology department, faculty of Medicine -Zagazig University Anatomy and Embryology Department, Faculty of Medicine, Al-Baha University, Al-Baha, Saudi Arabia. | ||||
| 3Medical Biochemistry Department, Faculty of Medicine, Zagazig University. | ||||
| 4Forensic medicine and Toxicology ,Faculty of Medicine, Zagazig University, Zagazig, | ||||
| 5Human anatomy and Embryology, Faculty of medicine, Zagazig University Egypt | ||||
| Abstract | ||||
| hepatoprotective effect by inducing the antioxidant defense mechanism. Aim of the Study: To evaluate aging-related impacts on the hepatic structure and function and the possible mitigating role of MO extract, with clarifying the mechanistic role of autophagy in aged rat models. Material and Methods: Twenty four rats, aged 3 months, were assigned into 4 groups as following, group I: adult control, group II: administered MO leaf aqueous extract (50 mg/kg body weight) by nasogastric tube for 4 months, group III: received no treatment till age 20 months, and group IV: received no treatment till age 16 months, and then received MO leaf aqueous extract for 4 months. At the time of sacrificing, serum liver enzymes (aspartate aminotransferase and alanine aminotransferase) were measured and the hepatic specimens were processed for light and electron-microscopic assessment and analysis of phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), and mammalian target of rapamycin (mTOR) gene expression. Results: The aged rats revealed significant elevation of liver enzymes and hepatic malondialdehyde as well as reduction of hepatic glutathione content. Aging also inhibited autophagy via upregulation of autophagy inhibitory genes; PI3K, Akt, and mTOR. Immuno-histopathological evaluation of the hepatic tissue of aged rats displayed deteriorated cytoarchitecture in terms of hydropic degeneration, congested veins, and inflammatory infiltrates in addition to increased glial fibrillary acidic protein expression and decreased microtubule-associated protein 1A/1B-light chain 3 (LC3) expression. MO administration to the aged rats promoted significant amelioration of aging-induced hepatic dysfunction and tissue alterations, counteracted oxidative stress and enhanced autophagy via downregulation of PI3K, Akt and mTOR genes Conclusion: MO can reduce aging-induced structural and functional liver damage by combating oxidative stress, and stimulation of autophagy through downregulation of the PI3K/Akt/mTOR signaling pathway. | ||||
| Keywords | ||||
| Aging; autophagy; glial fibrillary acidic protein; moringa oleifera extract; hepatocytes; PI3K/Akt/mTOR pathway | ||||
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