Antimicrobial and Antibiofilm Activity of Gold Nanoparticles Biosynthesized Using Acinetobacter Baumannii Isolated from Wounds and Burns
Al-Abdeli, I., Al-Kalifawi, E. (2023). Antimicrobial and Antibiofilm Activity of Gold Nanoparticles Biosynthesized Using Acinetobacter Baumannii Isolated from Wounds and Burns. EKB Journal Management System, 90(1), 1274-1283. doi: 10.21608/ejhm.2023.282021
Intesar H. Al-Abdeli; Esam J. Al-Kalifawi. "Antimicrobial and Antibiofilm Activity of Gold Nanoparticles Biosynthesized Using Acinetobacter Baumannii Isolated from Wounds and Burns". EKB Journal Management System, 90, 1, 2023, 1274-1283. doi: 10.21608/ejhm.2023.282021
Al-Abdeli, I., Al-Kalifawi, E. (2023). 'Antimicrobial and Antibiofilm Activity of Gold Nanoparticles Biosynthesized Using Acinetobacter Baumannii Isolated from Wounds and Burns', EKB Journal Management System, 90(1), pp. 1274-1283. doi: 10.21608/ejhm.2023.282021
Al-Abdeli, I., Al-Kalifawi, E. Antimicrobial and Antibiofilm Activity of Gold Nanoparticles Biosynthesized Using Acinetobacter Baumannii Isolated from Wounds and Burns. EKB Journal Management System, 2023; 90(1): 1274-1283. doi: 10.21608/ejhm.2023.282021

Antimicrobial and Antibiofilm Activity of Gold Nanoparticles Biosynthesized Using Acinetobacter Baumannii Isolated from Wounds and Burns

The Egyptian Journal of Hospital Medicine
Article 191, Volume 90, Issue 1, January 2023, Page 1274-1283  XML PDF (1.15 MB)
Document Type: Original Article
DOI: 10.21608/ejhm.2023.282021
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Authors
Intesar H. Al-Abdeli email ; Esam J. Al-Kalifawi
Abstract
Background: Due to their biological and physiochemical characteristics, metal-based nanoparticles show promise as antimicrobials and therapeutic agents.
Aim: This study aimed to biosynthesize AuNPs from A. baumannii broth and test their antibacterial and ant-virulence properties.
Materials and methods: UV-vis, FTIR, XRD, TEM, FESEM and AMF were used to analyze biosynthesized gold nanoparticles. The well method diffusion assay was utilized to determine Ab-AuNPs' antibacterial activity against five bacterial strains. Microtiter plate was used to study the effect of Ab-AuNPs on biofilm formation in five bacterial isolates.
Results Biosynthesized Ab-AuNPs feature 574 nm UV-Visible SPR bands. Ab-AuNPs biosynthesized at 3321.42, 2823.79, 2144.84, 1631.78 cm-1, amide III, polypeptides, and proteins form these bands. A. baumannii polypeptides capped AuNPs. The peaks at 111, 200, 220, and 311 reflect the 2 Bragg angles 38.18°, 44.51°, 64.80°, and 77.72°. Debye Scherrer's equation calculated Ab-AuNPs crystallite size (26.82 nm). Biosynthesized Ab-AuNPs were 20-90 nm in size, with an average of 66 nm. AFM measures Ab-AuNP shape. AFM images show Ab-AuNP size distribution. Ab-AuNPs averaged 63.82 nm. The diameter of the inhibitory zone decreases with Ab-AuNP concentration. Biosynthesized Ab-AuNPs inhibited biofilm formation in five bacterial strains.
Conclusions: We concluded that the biosynthesized Ab-AuNPs have effective antibacterial and antibiofilm activity that could enhance the action of existing antibiotics and could be a therapeutic agent.
 
 
Keywords
Gold nanoparticles; Acinetobacter baumannii; Antibiofilm activity; Antibacterial activity
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