Study of mcr-1 Gene-Mediated Colistin-Resistance in Gram-Negative Isolates in Egypt
Emara, M., Abd-Elmonsef, M., Abo Elnasr, L., Elfeky, A. (2019). Study of mcr-1 Gene-Mediated Colistin-Resistance in Gram-Negative Isolates in Egypt. EKB Journal Management System, 28(3), 9-16. doi: 10.21608/ejmm.2019.282890
Manar M.M. Emara; Marwa M.E. Abd-Elmonsef; Lobna M. Abo Elnasr; Aziza Abo El-Enain Elfeky. "Study of mcr-1 Gene-Mediated Colistin-Resistance in Gram-Negative Isolates in Egypt". EKB Journal Management System, 28, 3, 2019, 9-16. doi: 10.21608/ejmm.2019.282890
Emara, M., Abd-Elmonsef, M., Abo Elnasr, L., Elfeky, A. (2019). 'Study of mcr-1 Gene-Mediated Colistin-Resistance in Gram-Negative Isolates in Egypt', EKB Journal Management System, 28(3), pp. 9-16. doi: 10.21608/ejmm.2019.282890
Emara, M., Abd-Elmonsef, M., Abo Elnasr, L., Elfeky, A. Study of mcr-1 Gene-Mediated Colistin-Resistance in Gram-Negative Isolates in Egypt. EKB Journal Management System, 2019; 28(3): 9-16. doi: 10.21608/ejmm.2019.282890

Study of mcr-1 Gene-Mediated Colistin-Resistance in Gram-Negative Isolates in Egypt

Egyptian Journal of Medical Microbiology
Volume 28, Issue 3, July 2019, Page 9-16  XML PDF (379.38 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2019.282890
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Authors
Manar M.M. Emara email 1; Marwa M.E. Abd-Elmonseforcid 2; Lobna M. Abo Elnasr3; Aziza Abo El-Enain Elfeky2
11Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt
2Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt
3Anaesthesia and Surgical Intensive Care Departments, Faculty of Medicine, Tanta University, Egypt
Abstract
Background: The re-use of colistin (last-resort drug) to treat infections caused by resistant Gram-negative bacteria, has led to the emergence of a serious resistance against colistin. A new transferable plasmid-mediated colistin-resistance gene (mcr-1) has been described globally. Screening for such gene will provide an aiding step to explore the extent of colistin-resistance in Egypt. Objectives: To isolate the causative Gram-negative bacteria from different hospital-acquired (HA) and community-acquired (CA) infections, determine the antibiotic susceptibility pattern of the isolated bacteria, detect colistin-resistance and investigate the existence of mcr-1 gene in colistin-resistant isolates. Methodology: This study was carried out on 400 patients with HA- and CA-infections. Samples were taken from sputum, endotracheal aspirates, bronchoalveolar lavage, surgical and burn wounds, stool and blood. Bacterial isolation and identification were done by standard microbiological methods. Colistin-resistance was assessed by broth macrodilution method, then mcr-1 gene was detected in colistin-resistant isolates by conventional polymerase chain reaction (PCR). Results: Gram-negative organisms were the commonest isolates in both HA (67.6%) and CA (79.4%) infections. Colistin-resistance was detected in only 10 cases. mcr-1 gene was not detected in any of the tested colistin-resistant isolates. Conclusion: The prevalence of colistin-resistance in the study cases is still low and has not extended to the community yet. Colistin intake is not a prerequisite for the occurrence of resistance, but could be a supporting factor.
Keywords
Colistin- resistance; mcr-1 gene; PCR
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