A Comparative Study of MALDI-TOF MS and VITEK2 for Identification of Aerobic Gram Positive and Yeast Strains Isolated in Clinical Microbiology Laboratory
Ibraheem, H., Fawzi, M., El- Gerby, M. (2019). A Comparative Study of MALDI-TOF MS and VITEK2 for Identification of Aerobic Gram Positive and Yeast Strains Isolated in Clinical Microbiology Laboratory. EKB Journal Management System, 28(3), 43-48. doi: 10.21608/ejmm.2019.283021
Hoda A. Ibraheem; Maggie M. Fawzi; Manal M. El- Gerby. "A Comparative Study of MALDI-TOF MS and VITEK2 for Identification of Aerobic Gram Positive and Yeast Strains Isolated in Clinical Microbiology Laboratory". EKB Journal Management System, 28, 3, 2019, 43-48. doi: 10.21608/ejmm.2019.283021
Ibraheem, H., Fawzi, M., El- Gerby, M. (2019). 'A Comparative Study of MALDI-TOF MS and VITEK2 for Identification of Aerobic Gram Positive and Yeast Strains Isolated in Clinical Microbiology Laboratory', EKB Journal Management System, 28(3), pp. 43-48. doi: 10.21608/ejmm.2019.283021
Ibraheem, H., Fawzi, M., El- Gerby, M. A Comparative Study of MALDI-TOF MS and VITEK2 for Identification of Aerobic Gram Positive and Yeast Strains Isolated in Clinical Microbiology Laboratory. EKB Journal Management System, 2019; 28(3): 43-48. doi: 10.21608/ejmm.2019.283021

A Comparative Study of MALDI-TOF MS and VITEK2 for Identification of Aerobic Gram Positive and Yeast Strains Isolated in Clinical Microbiology Laboratory

Egyptian Journal of Medical Microbiology
Volume 28, Issue 3, July 2019, Page 43-48  XML PDF (229.55 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2019.283021
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Authors
Hoda A. Ibraheem email ; Maggie M. Fawzi; Manal M. El- Gerby
Department of Clinical Pathology, Faculty of Medicine, Zagazig University, Egypt
Abstract
Background: Matrix-assisted laser desorption ionization- time of flight mass spectrometry (MALDI-TOF MS) has recently been used in microbiology laboratories for rapid and accurate identification of bacterial and yeast strains isolated from clinical samples. Objective: To evaluate the analytical and practical performance of MALDI -TOF MS (VILEK MS) system in comparison to conventional VITEK2 system for identification of aerobic gram positive and yeast strains. Methodology: A total of 416 isolates (340 aerobic Gram positive bacteria and 76 yeast strains) representing 5 genera and 30 species were analysed. Unmatched results were further investigated by 16s rRNA gene sequencing according to a reference method. Results: For 416 isolates, All strains (100%) were accurately identified at the genus level and 409 (98.3%) isolates at species level by using MALDI TOF MS. A total of 388 (93.26%) isolates were completely matched by both methods. There were 28 unmatched isolates, MALDI TOF MS made no errors at the genus level while VITEK 2 made 4 (0.96%) errors at the same level. At the species level the identification error rates of MALDI -TOF MS and VITEK 2 were 1.68% and 5.04% respectively. Conclusion: our findings demonstrated that MALDI -TOF MS has better performance than VITEK 2 in microbial identification. It is accurate, quick and cost- effective technique for bacterial identification in the Clinical Microbiology Laboratory.
Keywords
MALDL-TOF MS; gram positive; yeast strains identification
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