Role of HLA-G 14 bp polymorphism and soluble HLA-G level in recurrent spontaneous abortion
El Shahaway, A., Salah, W., Shazly, S., Abd Elhady, R. (2019). Role of HLA-G 14 bp polymorphism and soluble HLA-G level in recurrent spontaneous abortion. EKB Journal Management System, 28(4), 127-132. doi: 10.21608/ejmm.2019.283355
Alia A. El Shahaway; Wafaa Salah; Sherin A. Shazly; Rasha R. Abd Elhady. "Role of HLA-G 14 bp polymorphism and soluble HLA-G level in recurrent spontaneous abortion". EKB Journal Management System, 28, 4, 2019, 127-132. doi: 10.21608/ejmm.2019.283355
El Shahaway, A., Salah, W., Shazly, S., Abd Elhady, R. (2019). 'Role of HLA-G 14 bp polymorphism and soluble HLA-G level in recurrent spontaneous abortion', EKB Journal Management System, 28(4), pp. 127-132. doi: 10.21608/ejmm.2019.283355
El Shahaway, A., Salah, W., Shazly, S., Abd Elhady, R. Role of HLA-G 14 bp polymorphism and soluble HLA-G level in recurrent spontaneous abortion. EKB Journal Management System, 2019; 28(4): 127-132. doi: 10.21608/ejmm.2019.283355

Role of HLA-G 14 bp polymorphism and soluble HLA-G level in recurrent spontaneous abortion

Egyptian Journal of Medical Microbiology
Volume 28, Issue 4, October 2019, Page 127-132  XML PDF (413.2 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2019.283355
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Authors
Alia A. El Shahaway email 1; Wafaa Salah2; Sherin A. Shazly2; Rasha R. Abd Elhady2
1Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
2Obstetrics and gynecology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Abstract
Background: The immune response of the mother against her embryo is supposed to be responsible for about 50 % of recurrent spontaneous abortion (RSA) case. Objectives: To assess the prevalence of HLA-G 14bp insertion/deletion (ins/del) polymorphism in females with RSA and normal pregnant females and compare the plasma levels of soluble HLA-G (sHLA-G) in the studying groups. Also, we intended to explore the association between HLA-G 14 bp polymorphism and sHLA-G plasma levels. Methodology: This case-control study involved 50 females with RSA and 50 control pregnant females. The genotype for HLA-G 14 bp (ins/del) polymorphism was performed by PCR and their sHLA-G plasma levels were measured. Results: The HLA-G del/del genotype and ins/ins genotype frequencies were significantly different in RSA group as compared to controls (P=0.002). Additionally, the incidence of the 14-bp ins allele was significantly raised in RSA group than in control (67 vs. 41%, respectively; P=0.0004). Consequently, the higher frequency of 14-bp ins allele in RSA group as compared to controls indicate that this allele is associated with an increased risk of RSA (OR 2.9, 95% CI: 1.6-5.2, P=0.0003). Conclusion: The plasma level of sHLA-G is a promising marker in the management of RSA and could be an early indicator of the fate of In Vitro Fertilization (IVF). The polymorphism in the HLA-G gene, specifically, in the 3′UTR region of exon 8 affects the plasma level of sHLA-G and subsequent pregnancy outcome.
Keywords
Recurrent spontaneous loss; HLA-G 14bp in/del; exon 8; HLA-G gene; HLA-G genotype
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