VITAMIN D AND VITAMIN D RECEPTOR GENE VARIANT IN EGYPTIAN MULTIPLE SCLEROSIS PATIENTS: A CASE-CONTROL STUDY
Sultan, D., Mandour, I., Geba, K., Khallaf, A., Radwan, N. (2023). VITAMIN D AND VITAMIN D RECEPTOR GENE VARIANT IN EGYPTIAN MULTIPLE SCLEROSIS PATIENTS: A CASE-CONTROL STUDY. EKB Journal Management System, 9(1), 46-52. doi: 10.21608/jbaar.2023.285279
Doaa Mohammed Sultan; Iman A. Mandour; Khaled Mohamed Geba; Asmaa Galal- Khallaf; Noha A. Radwan. "VITAMIN D AND VITAMIN D RECEPTOR GENE VARIANT IN EGYPTIAN MULTIPLE SCLEROSIS PATIENTS: A CASE-CONTROL STUDY". EKB Journal Management System, 9, 1, 2023, 46-52. doi: 10.21608/jbaar.2023.285279
Sultan, D., Mandour, I., Geba, K., Khallaf, A., Radwan, N. (2023). 'VITAMIN D AND VITAMIN D RECEPTOR GENE VARIANT IN EGYPTIAN MULTIPLE SCLEROSIS PATIENTS: A CASE-CONTROL STUDY', EKB Journal Management System, 9(1), pp. 46-52. doi: 10.21608/jbaar.2023.285279
Sultan, D., Mandour, I., Geba, K., Khallaf, A., Radwan, N. VITAMIN D AND VITAMIN D RECEPTOR GENE VARIANT IN EGYPTIAN MULTIPLE SCLEROSIS PATIENTS: A CASE-CONTROL STUDY. EKB Journal Management System, 2023; 9(1): 46-52. doi: 10.21608/jbaar.2023.285279

VITAMIN D AND VITAMIN D RECEPTOR GENE VARIANT IN EGYPTIAN MULTIPLE SCLEROSIS PATIENTS: A CASE-CONTROL STUDY

Journal of Bioscience and Applied Research
Volume 9, Issue 1, March 2023, Pages 46-52    PDF (986.55 K)
Document Type: Original Article
DOI: 10.21608/jbaar.2023.285279
Authors
Doaa Mohammed Sultan1; Iman A. Mandour2; Khaled Mohamed Geba3; Asmaa Galal- Khallaf3; Noha A. Radwan* 2
1Department of Zoology Chemistry, Faculty of Science, Ain Shams University, Egypt
2Clinical and Chemical Pathology, Kasr Alainy Medical School, Cairo University, Egypt
3Molecular Biology Department, Faculty of Science, Monifeya University, Egypt
Abstract
Aim: Multiple sclerosis (MS) is an autoimmune disease with a controversial etiology. Both genetic and environmental factors are thought to be involved in the risk of developing the disease. The purpose of this study was to assess the association of the Vitamin D receptor (VDR) BsmI variant with MS and to investigate the interaction of this variant with vitamin D levels. Method: 100 subjects were recruited for this study. Fifty patients were diagnosed with MS and 50 were healthy individuals. BsmI was genotyped by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analyses in both groups and serum 25-hydroxyvitamin D [25(OH)D] levels were determined in MS patients by high-performance liquid chromatography (HPLC). Results: The distribution of the genotype of VDR polymorphism BsmI did not differ significantly between MS patients and healthy controls. The G allele of BsmI was a statistically significant higher percentage in MS patients (p-value 0.045). There is no statistically significant difference in the level of 25(OH)D between MS patients and the control group. Conclusions: The study findings suggest that the VDR gene variant BsmI G allele may increase the risk of the development of MS.
Keywords
VDR gene; RFLP; Vitamin D - HPLC
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