Evaluation of CD27 Expression on Mycobacterial Antigen-Specific CD4+ T Cells as an Immunological Marker for Diagnosis of Active Pulmonary Tuberculosis
Gadallah, M., Elanany, A., Abd Elwahab, M., El Shaffey, B., El-Naghy, W., El-dib, A., Sharawy, D., Dahy, N., Singh, M., Scientific Research Group, L. (2018). Evaluation of CD27 Expression on Mycobacterial Antigen-Specific CD4+ T Cells as an Immunological Marker for Diagnosis of Active Pulmonary Tuberculosis. EKB Journal Management System, 27(2), 71-79. doi: 10.21608/ejmm.2018.285544
Mona Abd El-Aziz Gadallah; Abo Elfetoh E. Elanany; Marwa A. Abd Elwahab; Basem I. El Shaffey; Wageih S. El-Naghy; Ayman S. El-dib; Dalia El Sharawy; Nadia M. Dahy; Mahavir Singh; LIONEX Scientific Research Group. "Evaluation of CD27 Expression on Mycobacterial Antigen-Specific CD4+ T Cells as an Immunological Marker for Diagnosis of Active Pulmonary Tuberculosis". EKB Journal Management System, 27, 2, 2018, 71-79. doi: 10.21608/ejmm.2018.285544
Gadallah, M., Elanany, A., Abd Elwahab, M., El Shaffey, B., El-Naghy, W., El-dib, A., Sharawy, D., Dahy, N., Singh, M., Scientific Research Group, L. (2018). 'Evaluation of CD27 Expression on Mycobacterial Antigen-Specific CD4+ T Cells as an Immunological Marker for Diagnosis of Active Pulmonary Tuberculosis', EKB Journal Management System, 27(2), pp. 71-79. doi: 10.21608/ejmm.2018.285544
Gadallah, M., Elanany, A., Abd Elwahab, M., El Shaffey, B., El-Naghy, W., El-dib, A., Sharawy, D., Dahy, N., Singh, M., Scientific Research Group, L. Evaluation of CD27 Expression on Mycobacterial Antigen-Specific CD4+ T Cells as an Immunological Marker for Diagnosis of Active Pulmonary Tuberculosis. EKB Journal Management System, 2018; 27(2): 71-79. doi: 10.21608/ejmm.2018.285544

Evaluation of CD27 Expression on Mycobacterial Antigen-Specific CD4+ T Cells as an Immunological Marker for Diagnosis of Active Pulmonary Tuberculosis

Egyptian Journal of Medical Microbiology
Volume 27, Issue 2, April 2018, Pages 71-79    PDF (604.81 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2018.285544
Authors
Mona Abd El-Aziz Gadallah* 1; Abo Elfetoh E. Elanany2; Marwa A. Abd Elwahab2; Basem I. El Shaffey3; Wageih S. El-Naghy2; Ayman S. El-dib3; Dalia El Sharawy3; Nadia M. Dahy2; Mahavir Singh4; LIONEX Scientific Research Group4
1Medical microbiology and immunology department,faculty of medicine,Tanta university,Egypt
2Medical Microbiology & Immunology Department, Faculty of Medicine, Tanta University
3Department of Chest diseases, Faculty of Medicine, Tanta University
4Lionex Diagnostics and Therapeutics, SalzdahlumerStraße 196, 38126 Braunschweig, Germany
Abstract
Background: Until now, there is a need for novel tools helping the prompt diagnosis tuberculosis (TB). Objective: This study aims to explore the extent of CD27 expression on ESAT-6 & CFP-10 antigen-specific CD4+ T cells as a recent biomarker for rapid diagnosis of active pulmonary TB in Egypt. Methodology: Based on IFN-γ+ expression, ESAT-6 & CFP-10 antigen-specific CD4+ T cells were identified and the CD27 expression was analyzed for 60 M. tuberculosis-infected patients and 20 healthy controls. Results: The present data, in fact, displayed that patients with pulmonary TB had significantly lowered CD27 expression than healthy controls (P <0.001). The persistent active TB patients had much lesser percentages of CD27+ T cells than culture-positive recently infected TB patients (P =0.014) and healthy controls (P< 0.001). On evaluating the diagnostic performance of CD27 expression, it has been declared that at a cutoff value of 43.64%. CD27 had a sensitivity, specificity, positive predicted value (PPV), and negative predicted value (NPV) 86.7%, 100%, 100%, 71.4% respectively, for discrimination between patients with TB from healthy subjects. Conclusion: Thereby, proportion of CD27+ antigen-specific CD4 T cells could be used as an immunological marker for active TB.
Keywords
Tuberculosis; flow cytometry; CD4+ T cells; CD27; ESAT-6 & CFP-10 antigens
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