Validation of GeneXpert MRSA/SA Blood Culture Assay in Patients with Suspected Bacteremia | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 27, Issue 3, July 2018, Pages 1-6 PDF (234.07 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2018.285581 | ||
| Author | ||
| Sarah M. Abdelhamid* | ||
| Microbiology and Immunology Department, Faculty of Pharmacy, Damanhour University | ||
| Abstract | ||
| Background: The GeneXpert MRSA/SA Blood Culture (BC) assay was validated prospectively in patients with suspected bacteremia. Objectives: The purpose of this study was to compare the Xpert MRSA/SA Blood Culture Assay to conventional culturing techniques. Methodology: Positive blood cultures flagged up by the BacT/ALERT® 3D system were gram stained and subcultured. Results: Out of the 33, 28 (84.8%) were identified as CoNs by phenotypic methods. The remaining 5 were identified as S aureus. GeneXpert results were concordant with the phenotypic results in 30 (90.9%) of samples. Twenty two of the CoNs were methicillin-resistant isolates and five were methicillin-sensitive isolates. Of the 5 S aureus , 3 were concordant. One MRSA and two MSSA. This result is statistically significant at p < .05. The mean time to notification of Xpert BC assay results was 1.5 hours. Conclusion: The Xpert assay is dependable, fast and reproducible for on-demand testing in health care settings where such testing is needed. | ||
| Keywords | ||
| GeneXpert; MRSA; BacT/ALERT; Blood Culture | ||
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