DIFFERENTIATION AMONG RALSTONIA SOLANACEARUM ISOLATES CAUSING POTATO BACTERIAL WILT BY RANDOM AMPLIFIED POLYMORPHIC DNA | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 2, Volume 81, Issue 4, December 2003, Page 1505-1520 PDF (3.59 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2003.287181 | ||||
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| Authors | ||||
| MAGGIE E. M. HASSAN; MOHAMED S. KHALIL; NABIL S. FARAG; SHERIEN A. HARPHOUSH | ||||
| Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt | ||||
| Abstract | ||||
| Ten Ralstonia solanaceannn isolatos were obtained from Spunta and Nicola potato cultivars . PCR-based RAPD analysis was carried out, using four primers, on agarose and polyacrylamide gels as support media. Only, the primer 6-d (AACGCGCAAC)-3 differentiated the Isolates recovered from the two cultivars, others did not. In general, separation on poly-acrylamide and staining with silver nitrate gave better resolution than those on agarose and stained with ethidium bromide. Further investigations are needed using greater number of :solates, from different potato cultivars and /or different sources, to confirm the result as well as the possible effect of the host cultivars on the properties of bacterial DNA. | ||||
| Keywords | ||||
| Ralstonia solanacearum; potato cultivars; polyacrylamide; silvor nitrate and RAPD | ||||
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