Detection of flaA, fliC , mrpA and rsbA Gene in proteus mirabilis Multidrug Resistance Isolated from Different Clinical Sources in Baquba City
AL-Dulaimy, I., Saleem, A., Al-Taai, H. (2023). Detection of flaA, fliC , mrpA and rsbA Gene in proteus mirabilis Multidrug Resistance Isolated from Different Clinical Sources in Baquba City. EKB Journal Management System, 90(2), 2831-2838. doi: 10.21608/ejhm.2023.287337
Ibraheem M. AL-Dulaimy; Ali Jaffar Saleem; Hadi R. Rasheed Al-Taai. "Detection of flaA, fliC , mrpA and rsbA Gene in proteus mirabilis Multidrug Resistance Isolated from Different Clinical Sources in Baquba City". EKB Journal Management System, 90, 2, 2023, 2831-2838. doi: 10.21608/ejhm.2023.287337
AL-Dulaimy, I., Saleem, A., Al-Taai, H. (2023). 'Detection of flaA, fliC , mrpA and rsbA Gene in proteus mirabilis Multidrug Resistance Isolated from Different Clinical Sources in Baquba City', EKB Journal Management System, 90(2), pp. 2831-2838. doi: 10.21608/ejhm.2023.287337
AL-Dulaimy, I., Saleem, A., Al-Taai, H. Detection of flaA, fliC , mrpA and rsbA Gene in proteus mirabilis Multidrug Resistance Isolated from Different Clinical Sources in Baquba City. EKB Journal Management System, 2023; 90(2): 2831-2838. doi: 10.21608/ejhm.2023.287337

Detection of flaA, fliC , mrpA and rsbA Gene in proteus mirabilis Multidrug Resistance Isolated from Different Clinical Sources in Baquba City

The Egyptian Journal of Hospital Medicine
Article 126, Volume 90, Issue 2, January 2023, Page 2831-2838  XML PDF (848.5 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2023.287337
View on SCiNiTO View on SCiNiTO
Authors
Ibraheem M. AL-Dulaimy email ; Ali Jaffar Saleem; Hadi R. Rasheed Al-Taai
Abstract
Background: P. mirabilis possesses multiple virulence factors that enable them to cause infection in various anatomical sites of the human body, including endotoxin, and some of them are secreted into a medium such as the enzyme that analyzes urea, hemolytic enzyme, protease, biofilm, DNase, and others. Excessive use of antibiotics has led to the emergence of multidrug resistance and multidrug resistance bacteria that cause serious infections that are difficult to treat.
Objectives: The study aims to molecular investigation of the virulence genes in Proteus mirabilis multidrug resistant.
Methods: A total of 100 samples collected from different clinical sources (urinary tract infections, wounds, stool) in sterile containers in different hospitals in Baquba city (Baquba Teaching Hospital and Al-Batool Hospital) were subjected to antibiotic susceptibility study using disc-diffusion method. Molecular detection of virulence genes using polymerase chain reaction (PCR) analysis and amplicons sequenced.
Results: The results showed that all isolates had multiple resistance against antibiotic, and the highest resistance was against antibiotic Trimethoprim and lowest resistance towards two antibiotic Imipenem and Amikacin . PCR analysis revealed the presence of flaA ,fliC, mrp < /em>A and rsbA were present in all isolates in a ratio 100 %.
Conclusion: Isolates of Proteus mirabilis bacteria, which have virulence factors, it causes many diseases and susceptible to infection through their ability to move, transfer, adhere, and form a biofilm.
 
Keywords
Proteus mirabilis; urinary tract infection; virulence factor, rsbA gene
Statistics
Article View: 395
PDF Download: 625