Urinary Tract Infection Caused by Carbapenemase-Producing K. pneumoniae and E. coli at the Institute of Kidney Disease Peshawar, Pakistan
Hariri, S., Khan, S. (2023). Urinary Tract Infection Caused by Carbapenemase-Producing K. pneumoniae and E. coli at the Institute of Kidney Disease Peshawar, Pakistan. EKB Journal Management System, 15(1), 259-265. doi: 10.21608/eajbsc.2023.290749
Sumyya H. Hariri; Suleman Khan. "Urinary Tract Infection Caused by Carbapenemase-Producing K. pneumoniae and E. coli at the Institute of Kidney Disease Peshawar, Pakistan". EKB Journal Management System, 15, 1, 2023, 259-265. doi: 10.21608/eajbsc.2023.290749
Hariri, S., Khan, S. (2023). 'Urinary Tract Infection Caused by Carbapenemase-Producing K. pneumoniae and E. coli at the Institute of Kidney Disease Peshawar, Pakistan', EKB Journal Management System, 15(1), pp. 259-265. doi: 10.21608/eajbsc.2023.290749
Hariri, S., Khan, S. Urinary Tract Infection Caused by Carbapenemase-Producing K. pneumoniae and E. coli at the Institute of Kidney Disease Peshawar, Pakistan. EKB Journal Management System, 2023; 15(1): 259-265. doi: 10.21608/eajbsc.2023.290749

Urinary Tract Infection Caused by Carbapenemase-Producing K. pneumoniae and E. coli at the Institute of Kidney Disease Peshawar, Pakistan

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Volume 15, Issue 1, June 2023, Page 259-265  XML PDF (480.53 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2023.290749
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Authors
Sumyya H. Hariri1; Suleman Khan2
1Department of Microbiology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
2Department of Agricultural Sciences, Food, Natural Resources and Engineering, Università degli studi di Foggia, Italy
Abstract
Aims: Carbapenemase-producing bacteria make infections of the urinary tract (UTIs) challenging to cure with last-resort treatment like carbapenem. Carbapenemase-producing E. coli and K. pneumoniae implicated in UTI must be detected molecularly since their ability to spread broadly among patients is rising (Nomeh et al., 2022). Methodology: Ten non-repeated clinical isolates of Escherichia coli (Ecoli1, Ecoli2, Ecoli3, Ecoli4, and Ecoli5) and Klebsiella pneumoniae (Kp6, Kp7, Kp8, Kp9, Kp10) were selected from urinary tract infection patients at Institute of Kidney Disease Peshawar, Pakistan, based on their in vitro phenotypic carbapenem antibiotic resistance. These isolates were confirmed using standard routine microbiological techniques. PCR-specific primers screened E. coli and K. pneumoniae strains for Carbapenemase-producing genes. Result: Molecular Detection of Carbapenemase-producing Gene in UTI Patients with Uropathogenic Escherichia coli and Klebsiella pneumoniae. The higher proportion of Carbapenemase-producing genes in all the bacterial isolates in this study was blaKPC 15(100 %), followed by blaNDM 12.3(90.1 %), blaIMP 6(60.2 %) and blaVIM 3(30.6 %). The most common Carbapenemase gene in Escherichia coli 8 (80%) was blaKPC, followed by blaNDM 7 (70%) and blaOXA 45 (4.5%), which was the least common. Klebsiella pneumoniae had more blaNDM and blaKPC than blaOXA. Both had a percentage of 4.5 (40.9%). Conclusion: These results are consistent with the rapid spread of genes responsible for generating Carbapenemases in E. coli and Klebsiella pneumoniae that cause urinary tract infections. Despite the lack of blaVIM in K. pneumoniae, the pathogenic function of Carbapenemase-producing genes in UTI in this study should not be underestimated because of the potential they have to cause treatment failure and the subsequent persistence of UTI in patients.
Keywords
Urinary tract infection; Carbapenemase-producing; Escherichia coli, Klebsiella pneumoniae
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