Efficacy of Silver Nanoparticle-Coated Crude Extract of Aspergillus fumigatus Against Bacterial Infections from Surgical Sites Wound | ||||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
| Volume 15, Issue 1, June 2023, Page 305-311 PDF (499.96 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsc.2023.292071 | ||||
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| Author | ||||
| Nabeela Al-Abdullah | ||||
| King Abdulaziz University, Faculty of Nursing, Public Health Department, Infection Control Unit, King Abdulaziz University Hospital, Jeddah, Saudi Arabia | ||||
| Abstract | ||||
| The chemical reduction was used to make nanoparticles of silver. Different methods, such as UV-Vis absorption spectroscopy, were used to observe the silver nanoparticles being formed. The UV-spectroscopy showed that Plasmon absorption was between 408- and 420 mm. silver nanoparticles are made of molecules and are about 410 nm in size. We used X-ray diffraction (XRD), transmission electron microscopy (TEM), and UV–Vis spectroscopy (UV–Vis) to look at the nanoparticles. The X-ray diffraction analysis of the nanoparticle dispersion provided evidence that elemental silver was present in the sample. There were no peaks found that belonged to any other impurity. Transmission electron microscopy (TEM) was used to determine the consistent dimensions and morphology of silver nanoparticles appropriately. TEM image shows restricted size distribution (40 and 50 nm). The XRD pattern shows crystalline and cubic metallic silver peaks. The Kirby-Bauer method determined nanoparticle dispersion antibacterial activity. It was observed that silver nanoparticles possess potent antibacterial and bactericidal capabilities against gram-negative bacteria and gram-positive bacteria such as Escherichia coli, Pseudomonas aeruginosa, and staphylococcus aureus. When assessed on E. coli, the greatest antibacterial activity revealed a zone of inhibition measuring 17 mm. In contrast, it was observed that the antibacterial activity of ethyl crude extract at a concentration of 50 l was very low when it was tested against S.typhi (13mm). The highest antibacterial activity against E. coli was seen at 100 ml, which indicated a zone of inhibition measuring 23 mm. At a concentration of 100 ml ethyl acetate crude extract and AgNPs, a zone of inhibition measuring 25 mm was seen against S. typhi. While a zone of inhibition measuring 29 mm was seen against E. coli when 100 microliters of ethyl acetate crude extract and AgNPs were used, respectively. | ||||
| Keywords | ||||
| Nanoparticles silver; surface plasmons; ultraviolet-visible absorbance; fungal metabolites | ||||
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