The Beneficial Effects of Lipoic Acid Versus Coenzyme Q10 on Arginine-Induced Acute Pancreatitis in Albino Rats: Histological and Immunohistochemical study | ||||
| Journal of Medical Histology | ||||
| Article 5, Volume 5, Issue 2, December 2021, Page 154-170 PDF (2.71 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jmh.2022.161806.1103 | ||||
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| Authors | ||||
Rania Elsyade   1; Marwa Abdel Fattah2; Doaa Khaled 3; Doaa Eldosoki4
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| 1Department of Anatomy and Embryology, Faculty of Medicine, Helwan University, | ||||
| 2Department of Histology and Cytology, Misr University for Science and Technology | ||||
| 3Department of Histology and Cytology, Faculty of Medicine, Helwan University | ||||
| 4Department of Histology and Cytology, Faculty of Medicine, Fayoum University, Egypt. | ||||
| Abstract | ||||
| Background: Acute pancreatitis (AP) is a self-limiting condition to a widespread, deadly multi-organ failure that can result in death. Objectives: To compare the protective effect of alpha lipoic acid (ALA) and Coenzyme (Q10) in an experimental rat model of L-arginine-induced AP. Materials (Subjects): Forty adult male albino rats were randomized equally into 4 groups: (i) The control group; (ii) The AP group received L-Arginine by intraperitoneal (IP) injection in two doses of 2 g/kg body weight each, 1 hour apart, (iii) The ALA+AP group was injected with L-Arginine and intramuscular (IM) injection of ALA in two doses of 60mg/kg body weight; 1 hour before L-arginine injection and 24 hours after the second dose of L-arginine; (iii) The Coenzyme Q10+AP group was injected with L-Arginine and IM injection of Q10 in two doses of 30mg/kg body weight; 1 hour before L-arginine injection and 24 hours after the second dose of L-arginine. Histological, immunohistochemical for HSP70 and NF-κB, and morphometric studies were done followed by statistical analysis. Results: AP group showed significant distortion of the pancreatic acini with obvious cellular damage while those treated by ALA and Coenzyme Q 10 especially ALA showed significant pancreatic tissue improvement as shown by HSP70 and NF-κB immunoexpressing. Conclusions: ALA and Q 10 therapy proved a clear protective effect in suppressing experimentally generated AP in male albino rats, but the improvement was more obvious with ALA. | ||||
| Keywords | ||||
| Acute Pancreatitis; Coenzyme Q10; Lipoic Acid and HSP70; NF-kB | ||||
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