Optimization of hyaluronidase enzyme conditions produced from pseudomonas aerigenosa isolated from diabetic foot ulcer patients | ||
| Journal of Medical and Life Science | ||
| Volume 5, Issue 1, March 2023, Pages 23-30 PDF (333.79 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jmals.2023.295417 | ||
| Author | ||
| Furqan Majid Kadhum* | ||
| Department of Medical Optics Techniques, Al-Farabi University College, Baghdad, IRAQ | ||
| Abstract | ||
| Using clinically isolated pseudomonas aerigenosa, the best conditions for making hyaluronidase were studied. The production medium pH7 with 0.5% lactose and yeast extract, a novel bacterial culture (106Cell/ml), and 37oC for 24 hours produced the maximum hyaluronidase. The specific activity of the hyaluronidase that was generated in the culture medium rose to 130 U/mg of protein as a result of these circumstances. | ||
| Keywords | ||
| hyaluronidase; pseudomonas aerigenosa; culture medium | ||
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