Histological Study of the Possible Therapeutic Effect of Platelet Rich Plasma on the Healing of Second Degree Skin Burn and Prevention of Scar Formation in Adult Male Albino Rat | ||
| Egyptian Journal of Histology | ||
| Article 3, Volume 47, Issue 2, June 2024, Pages 646-670 PDF (5.24 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2023.193559.1858 | ||
| Authors | ||
| Asmaa Mohammed Mansour* 1; Mona Tayssir Sadek2; Essam mahmoud Laag3; Ebtsam Fouad Okasha4; Gehan Mohammed Soliman5 | ||
| 1Department of histology-faculty of medicine-Tanta university | ||
| 2Histology department, Faculty of medicine, Tanta University | ||
| 3Histology department,faculty of medicine tanta university | ||
| 4Histology department-Tanta Faculty of Medicine | ||
| 5Histology department, Faculty of Medicine, Tanta University | ||
| Abstract | ||
| Introduction: Burn is a skin injury causing hypertrophic scars. Platelet-rich plasma (PRP) represents a therapeutic method used for wound healing. Aim of the Work: To study the possible therapeutic effect of PRP on healing of second-degree skin burn and scar formation. Materials and Methods: This study was done on 70 adult male albino rats, randomly divided into three main groups; I, II & III. Group I included 30 rats that were randomly divided equally into three subgroups; I-A (for blood collection), I-B (control group kept without treatment) and I-C (control group which received a single subcutaneous injection of PRP, then skin specimens were obtained after 1week and 4weeks). Group II included 20 rats in which second-degree burn was induced by heated metal rod then subdivided into 2 equal subgroups; II-A (examined after 1week) and II-B (examined after 4weeks). Group III included 20 rats in which second-degree burn was induced then the rats were immediately treated locally by one subcutaneous injection of 0.4ml of PRP and further subdivided into two equal subgroups; III-A (examined after 1week) and III-B (examined after 4weeks). Skin specimens were examined using light microscopy (H&E, toluidine blue, Mallory’s trichrome, anti α SMA and CD34 immunostaining) and electron microscopy. Morphometric study and statistical analysis were done to measure mean epidermal thickness, mean collagen area percentage and mean color intensity of α SMA and CD34 immunoreaction. Results: In comparison to the untreated subgroups II-A and II-B, both treated subgroups III-A and III-B exhibited enhanced healing and accelerated scar maturation respectively that was more prominent after four weeks from PRP injection. Conclusion: PRP is effective in healing and scar maturation of second-degree skin burn. | ||
| Keywords | ||
| Skin burn; scar; PRP | ||
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