Histological Study on the Possible Protective Role of L-Carnitine Against Testicular Ischemia-Reperfusion Injury Induced in Rabbits | ||
| Egyptian Journal of Histology | ||
| Article 6, Volume 47, Issue 2, June 2024, Pages 694-705 PDF (1.13 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2023.199635.1873 | ||
| Authors | ||
| Mohamed Abdel Rahman1; Hala Madkour2; Fatma El-saeed El-Demerdash3; Hala Mahfouz4; Abdelrahman Ahmed Aburahma5; Sarah Mosaad Amer* 6 | ||
| 1Department of Human Anatomy and Embryology, Faculty of Medicine, Sohag University, Sohag, Egypt | ||
| 2Department of Pharmacology, Faculty of Medicine, Sohag University, Sohag, Egypt | ||
| 3Department of Zoology and Entomology, Faculty of Science (Girl's),Al-Azhar University, Cairo, Egypt | ||
| 4Department of medical biochemistry and molecular biology, Faculty of Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt | ||
| 5Department of Andrology, Faculty of Medicine, Cairo University, Manial, Cairo, Egypt | ||
| 6Department of Medical Histology and Cell Biology, Faculty of Medicine, Cairo University, Manial, Cairo, Egypt | ||
| Abstract | ||
| Introduction: The torsion of the testis is a surgical emergent situation which is common; however there are no effective measures to avoid damage of the testis after untwisting this torsion. L-carnitine (LC) is an essential micronutrient with strong anti-apoptotic, anti-inflammatory and antioxidant potencies that can reverse oxidative stress induced testicular damage. Aim of the Work: To evaluate the potential protective role of LC against the testicular damage induced by ischemia-reperfusion (IR) injury in rabbits. Materials and Methods: 50 adult male rabbits weighing 1300-1600 g were divided into 5 groups (10 rabbits per group). Acute IR testicular injury was induced in rabbits by twisting the left spermatic cord 720º for 2 h. Group 1: Control group and divided into two subgroups (subgroup A negative control and subgroup B positive control); Group 2: Sham-operated; Group 3: Ischemia group; Group 4: IR group (testicular IR was induced without LC); Group 5: IR+LC testicular IR was induced and LC was given by intra-peritoneal injection at a dose of 500 mg/kg immediately after torsion. At the experimental end, tissue samples of left testes were collected from all rabbits of the five groups and evaluated biochemically and histologically. Results: Ischemia-reperfusion resulted in disturbance of the oxidant/antioxidant status markers, loss of normal architecture of spermatogenic cells, apparent increase in the number of caspase-3 positive spermatogenic cells as well as abnormal fragmented chromatin material in addition to lack of halos around the sperm nuclei. However, the intra-peritoneal administration of LC immediately after torsion significantly improved these changes caused by IR on the testes. Conclusion: Administration of LC reduced the testicular damage induced by the ischemia-reperfusion. | ||
| Keywords | ||
| Ischemia-reperfusion injury; L-carnitine; rabbits; testes | ||
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