Ameliorative Effects of He:Ne Laser Irradiation on DNA Damage of Blood of Children with Acyanotic Heart Disease | ||||
| The International Conference on Mathematics and Engineering Physics | ||||
| Article 18, Volume 4, International Conference on Mathematics and Engineering Physics (ICMEP-4), May 2008, Page 1-13 PDF (318.92 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/icmep.2008.29896 | ||||
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| Authors | ||||
| Ekram Abdel Salam; Rabha El Shinawy; Soheir Korraa; Iman E. Abdel Meguid; Gihan El Sharnouby | ||||
| Abstract | ||||
| Abstract Low-energy laser irradiation (LELI) has been found to attenuate various biological processes in tissue culture and experimental animal models. The aim of the present study was to investigate the role of LELI in ameliorating markers of oxidative stress (in terms of blood antioxidant enzymes levels, NO generation, apoptotic genes together with DNA fragmentation and apoptosis percentage in circulating lymphocytes) in cyanotic and acyanotic congenital heart diseases with reference to normal healthy children. Accordingly, whole blood from 16 cyanotic, 14 acyanotic and 15 controls was exposed to 8 mW He-Ne laser at energy densities of 3 J/ cm2 and examined before, after and without laser exposure 24 hours post irradiation for levels blood antioxidant enzymes, NO generation, Bax RNA and Bcl2 protein together with DNA fragmentation and apoptosis percentage in circulating lymphocytes. Results indicated that there were significant increases in mean ± SD of plasma nitrite, MDA, DNA fragmentation and apoptosis in circulating lymphocytes of both acyanotic and cyanotic cases compared to controls before laser irradiation. Laser ameliorated oxidative stress in acyanotic heart disease blood, but increased apoptosis and DNA fragmentation in cyanotic cases 24 hours post laser irradiation. In control cases laser had a biostimulatory effect exhibited by decreased lipid peroxidation, apoptosis and DNA fragmentation compared to its level without laser irradiatioin. Also, there was significant increase in plasma nitrite in controls, cyanotic and acyanotic cases 24 hours post irradiation with laser compared to their level without laser irradiation. However, cyanotic cases showed the highest significant value and were significantly higher when compared to both cyanotic and acyanotic cases. It is concluded that He:Ne laser have an ameliorative effect on damaging effects induced due cardiac catheterization procedure in case of acyanotic congenital heart diseases, but it worsen the effects in cyanotic congenital heart diseases. Increased nitric oxide production seems to be the reason for the increased oxidative stress in cyanotic heart diseases, which are worsened when exposed to laser irradiation. | ||||
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