Effect of Abscisic acid on Legume Growth Rate, Seed Development and Protein Profile in Pisum sativum L. | ||
| Egyptian Journal of Botany | ||
| Article 19, Volume 63, Issue 3, September 2023, Pages 969-980 PDF (2.45 M) | ||
| Document Type: Regular issue (Original Article) | ||
| DOI: 10.21608/ejbo.2023.202685.2295 | ||
| Authors | ||
| Nada A. El-Taweel1; Mervat Sh. Sadak2; Noha S. Khalifa* 1 | ||
| 1Molecular Genetic Unit, Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt | ||
| 2Botany Department, Agricultural and Biological Institute, National Research Center, Dokki, P.O. 12622, Giza, Egypt | ||
| Abstract | ||
| In this work, cultivated pea (Pisum sativum L.cv. Master B) is used as a legume model crop plant to investigate the effect of abscisic acid (ABA) treatment on seed filling during embryogenesis. This was evaluated by using two exogenously applied concentrations (10& 20 µM) during three stages of seed developments (early, intermediate and late). Phenotypic characterization of the treated plants included measurement of seed / legume length and diameter at full maturation stages after treatment. The expression of major proteins of the treated plants was detected using SDS-PAGE electrophoresis technique. Our data showed a positive effect of ABA when applied at the late stage of seed development. ABA significantly increased legume size and elevated the expression of some major proteins such as convicilin (70-75 kDa), vicilin (50, 34 kDa) and legumin (40 kDa). The impact of these results is further discussed. | ||
| Keywords | ||
| ABA; Embryogenesis; Pisum sativum; SDS- PAGE; Seed filling | ||
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