Novel Anti-microbials from Streptomyces albidoflavus SAC61 against Multidrug-Resistant Bacteria: Metabolomics and Molecular Modeling | ||||
| Advances in Environmental and Life Sciences | ||||
| Article 4, Volume 4, Issue 2, October 2023, Page 29-38 PDF (1.35 MB) | ||||
| Document Type: Original research articles | ||||
| DOI: 10.21608/aels.2023.215761.1035 | ||||
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| Authors | ||||
Hanan Khaled Megawer   1, 2; Rabab Makharitta2, 3; Mohamed S. Nafie4; Raúl Riesco5; Martha E Trujillo5; Amro Hanora6; Sahar A El-Shatoury 2
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| 1Botany and Microbiology Department, Faculty of Science, Derna University, Algoba, Libya | ||||
| 2Botany and Microbiology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt | ||||
| 3Department of Biology, College of Sciences and Arts, University of Jeddah, 21959, Khulis, Jeddah, Saudi Arabia | ||||
| 4Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt | ||||
| 5Dpto. Microbiologíay Genética Lab. 214, University of Salamanca, 37007, Salamanca, Spain | ||||
| 6Microbiology Department, Faculty of Pharmacy, Suez Canal University, 41522, Ismailia, Egypt | ||||
| Abstract | ||||
| Antibiotic resistance developing bacteria have led to a renewed interest in bioactive compounds, particularly antibiotics. The combination of Actinomycetes and their bioactive metabolites with scientists using advanced techniques such as genomics and metabolomics to identify new compounds with potential as antibiotics is a promising combination. This study tested metabolic organic extracts from 29 actinomycete strains against four human pathogenic bacterial reference cultures. The results highlighted the bioactivity of Streptomyces albidoflavus SAC61, with the lowest MIC as a potential source of novel antimicrobial agents against Pseudomonas aeruginosa and Escherichia coli. Non-targeted metabolomics and molecular modeling technique (LC-QTOF-MS-MS) were used to identify potential mechanisms of action and target sites of the produced antibiotic. This study highlights the potential of Streptomyces albidoflavus SAC61 strain extracts as a good source of potent antimicrobial products. It emphasizes the need for further research to isolate and identify more active compounds and recognizes their mechanisms of action to combat antibiotic resistance. | ||||
| Keywords | ||||
| Actinomycetes; bioactive compounds; antibiotic resistance; Streptomyces albidoflavus | ||||
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