Polyphenolic Profiling and Analgesic Activity of Citrullus lanatus var. citroides Fruit | ||||
| Journal of Advanced Pharmacy Research | ||||
| Article 1, Volume 7, Issue 3, July 2023, Page 127-132 PDF (269.76 K) | ||||
| Document Type: Research Article | ||||
| DOI: 10.21608/aprh.2023.217966.1221 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Heba Khazaal1; Reham Bassyoni 1; Mokhtar Bishr2; Elsayed El-Sayed 3; Rabab El Dib1; Hesham Soliman  4, 1
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| 1Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Ain-Helwan, Cairo 11795, Egypt. | ||||
| 2Plant General Manager and Technical Director of the Arab Company for Pharmaceuticals and Medicinal Plants, Cairo, Egypt. | ||||
| 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Helwan University, Ain-Helwan, Cairo 11795, Egypt. | ||||
| 4Egypt Japan University for Science and Technology (EJUST), PharmD program. P. O. Box 179 New Borg El-Arab City, Alexandria, Egypt. | ||||
| Abstract | ||||
| Objectives: This study was designed for preliminary phytochemical screening of Citrullus lanatus var. citroides fruit pulp secondary metabolites. In addition, the polyphenolic compounds of the methanolic extract (ME) and its n-butanol soluble fraction (n-BF) were analyzed, and the in vivo analgesic activity of the richest polyphenolic fraction was evaluated. Methods: TheMEwas phytochemically screened for various secondary metabolites. Moreover, ME and n-BF polyphenolic profiling was performed using high performance liquid chromatography (HPLC) against external standards. The analgesic activity of the polyphenolic rich fraction (n-BF) was evaluated in vivo using acetic acid-induced abdominal writhing and hot plate tests. Results: Preliminary phytochemical screening revealed the presence of carbohydrate and /or glycosides, flavonoids, saponins, tannins, and unsaturated sterols and /or triterpenes, while alkaloids and/or compounds containing nitrogenous bases, anthraquinones, cardiac glycosides, and coumarins were absent. HPLC polyphenolic profiling showed the tentative identification of 11 phenolic compounds in ME (19.6959%), including 6 phenolic acids and 5 flavonoids, with gallic acid and rutin being the major identified ones; respectively. As well as, 17 phenolic compounds were tentatively identified in the n-BF (75.0771%), including 10 phenolic acids with chlorogenic acid as the major identified one, and 7 flavonoids with naringenin as the major one.Then-BF showed significant peripheral and central analgesic activity in a dose-dependent manner (p < 0.05), with n-BF at a dose of 1000 mg/kg being the most potent. Conclusion: The present study revealed that the n-BF was highly rich in phenolic compounds. These phenolic compounds were reported for the first time in C. lanatus var. citroides. The n-BF showed significant analgesic activity which was evaluated for the first time for the plant. | ||||
| Keywords | ||||
| Analgesic activity; Citrullus lanatus var. citroides; HPLC- Phytochemical screening; Polyphenols | ||||
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