Prevalence of Feline Panleukopenia Virus in Cats in Duhok Province, Iraq | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Volume 54, Issue 5, September and October 2023, Page 917-926 PDF (1.43 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2023.210719.1500 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Merna H. Dishow 1; Qaes Talb Alsarhan  2; Assel A. Al-Naqshabendy 3
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| 1Department of Medicine and Surgery- College of Veterinary Medicine- University of Duhok-Iraq. | ||||
| 2College of Veterinary Medicine, University of Mosul | ||||
| 3Department of Medicine and Surgery- College of Veterinary Medicine- University of Duhok-Iraq | ||||
| Abstract | ||||
| The current study targeted evaluation the prevalence of feline panleukopenia virus (FPV) in cats in Duhok province-Iraq, using immunochromatography assay (ICA) as a rapid test, indirect enzyme immunosorbent assay (i-ELISA) and conventional polymerase chain reaction (c-PCR). Moreover, determining the compatibility, sensitivity, specificity and accuracy between the different diagnostic techniques. A total of 100 fecal swab samples were collected from 52 household cats and 48 stray cats in various regions in Duhok province and tested using Rapid test and c-PCR, also 100 blood samples were drawn from cephalic and/or saphenous veins of same cats and tested using i-ELISA. The overall prevalence of FPV was 40%, 66% and 70% using rapid test, i-ELISA and c-PCR technique respectively. The prevalence was significantly higher in stray cats compared with household cats according to all tests used in this study. A moderate compatibility observed between rapid test and c-PCR based on Kappa value (0.440) with sensitivity 57.14%, specificity 100% and accuracy 70% of rapid test compared with c-PCR. Moreover, Fair compatibility between i-ELISA and c-PCR technique based on Kappa value (0.312) with sensitivity 75.71% , specificity 56.66%, and accuracy 70% of i-ELISA compared with c-PCR technique. It has been conclude that FPV is widespread in cats at Duhok province- Iraq. However, stray cats have a significant role in spreading of disease, and the rapid test and i-ELISA need to confirm using c-PCR technique. | ||||
| Keywords | ||||
| Feline Panleukopenia virus; ICA rapid test; i-ELISA; c-PCR; Duhok-Iraq | ||||
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