Comparative Study Between Traditional Methods, Commercial Biochemical Test and PCR for Identification of Campylobacter Species Isolated from Poultry Farms | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Article 12, Volume 28, Issue 1, June 2023, Pages 157-166 PDF (825.01 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2023.307787 | ||
| Authors | ||
| Mahmoud El Sayed1; Abo Elkheir Esawy2; Mona Sobhey3; Nada Eidaroos1; Rania Khattab* 2; Tamer ELfeky2; Mahmoud Abdelrahman2 | ||
| 1Bacteriology, Immunology and Mycology Department, Faculty of Veterinary medicine, Suez Canal University | ||
| 2Animal Health Research Institute, Mansoura Lab. Agriculture Research Center (ARC) | ||
| 3Animal Reproduction Research institute. | ||
| Abstract | ||
| Campylobacter spp < /em>. causes severe issues in chickens. In this investigation. Campylobacter species were isolated (182) in Dakahlia Governorate from 440 broilers and 440 ballade breeds. Campylobacter coli 42 isolates(4%)and Campylobacter jejuni140isolates (16%). For C. jejuni, rates of recovery from various internal organs were 60%, 24%, 10%, and 5%, from the cloacal swab, heart, liver, and gizzard respectively. The morphological studies revealed that the majority of species from Campylobacter are motile, survive at 37-42Co, and are G-ve, slender spirally curled. They require 5%O2, 85%N2, and 10%CO2 to create a colony that looks like a dew drop on mCCDA media. By using API20, the suspected isolates were identical to typical Campylobacter | ||
| Keywords | ||
| Campylobacter jejuni; C. coli; Poultry; API 20E and PCR | ||
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