EFFECT OF GINKGO BILOBA LOADED CHITOSAN SCAFFOLD ON HEALING OF BONE DEFECTS IN RABBIT | ||
| Alexandria Dental Journal | ||
| Article 4, Volume 48, Issue 2, August 2023, Pages 25-30 PDF (883.99 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/adjalexu.2022.128039.1264 | ||
| Authors | ||
| Doha Mohammed El-Sayed* 1; Amel R. El-Hak2; Dina A. Nagui3; Hossam El-Din M. Mostafa4 | ||
| 1Oral biology department, Faculty of Dentistry, Alexandria University, Egypt | ||
| 2Professor of Oral Biology -Faculty of Dentistry –Alexandria University | ||
| 3Assistant professor of Oral Biology- Faculty of Dentistry - Alexandria University | ||
| 4Lecturer of Oral Biology- Faculty of Dentistry - Alexandria University | ||
| Abstract | ||
| ABSTRACT Introduction: Critical sized bone defects do not heal completely by themselves and require intervention by using bone grafts or scaffolds. Chitosan (CS) is a widely used biomaterial for bone regeneration. Ginkgo biloba (GB) leaf’s extract is thought to be effective in stimulating bone cells differentiation. Polylactic-co-glycolic acid (PLGA) nanoparticles are commonly used as drug delivery vehicles. The PLGA nanoparticles were loaded with GB and incorporated in CS sponge scaffold. Objectives: To evaluate the effect of GB loaded PLGA nanoparticles incorporated in CS scaffold on healing of critical sized bone defects in rabbit. Materials and Methods: eighteen adult male New Zealand rabbits were randomly divided into 3 equal groups (six rabbits each): Group A (Control group), Group B (CS group) and Group C (GB+PLGA+CS group). Critical sized bone defects were performed in the edentulous area of rabbit mandibles (diastema). Group A: defects were left empty, Group B: defects were filled with unloaded CS scaffold, while in group C: defects were filled with CS scaffold incorporated with GB loaded PLGA nanoparticles. Animals were euthanized four weeks after surgery and the results were evaluated histologically and histomorphometrically. Results: GB loaded scaffold had a great effect on promoting regeneration of bone within the defects. Conclusion: the GB loaded CS scaffold is effective in bone regeneration and the GB extract has an osteoinductive potential. Keywords: Ginkgo biloba, PLGA nanoparticles, Chitosan, Bone defect, Bone regeneration Running title: Effect of GB-loaded CS scaffold on bone regeneration. | ||
| Keywords | ||
| Ginkgo biloba; PLGA nanoparticles; Chitosan; Bone defect; Bone regeneration | ||
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