Bacteriological and molecular studies on toxigenic Clostridium perfringens in milk and some milk products | ||
| Benha Veterinary Medical Journal | ||
| Article 19, Volume 31, Issue 2, December 2016, Pages 144-148 PDF (314.42 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2016.31283 | ||
| Authors | ||
| Ashraf A. Abd El Tawab1; Fatma I. El-Hofy1; Ahmed M. Ammar2; Hoda A. Aideia3; Eman A. Hammad4 | ||
| 1Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med. Benha Univ. | ||
| 2Microbiology Dept., Fac. Vet. Med. Zagazig Univ. | ||
| 3Animal Health Research Institute Dokki, Giza | ||
| 4General Authority for Vet. Services | ||
| Abstract | ||
| Two hundred random samples of milk, kareish cheese, yoghurt and ice-cream (50 for each) were examined microbiologically for the presence of Clostridium perfringens, their enterotoxigencity and their antibiotic sensitivity. Clostridium perfringens was isolated from 3 (6%) milk samples, 4 (8%) kareish cheese samples and it could not be isolated from any examined samples of yoghurt and ice-cream. The majority of C. perfringens isolates recovered from milk and milk products were susceptible to ofloxacin, ampicillin + sulbactam and norfloxacin (100%), vancomycin, tetracycline, metronidazole and amoxicillin + clavulinic acid (83.3%) and clindamycin (66.7%). The majority were resistant to cephalothin (100%), sulphamethoxazole + trimethoprim (83.3%), oxacillin and chloramphenicol (66.7%). Molecular studies using multiplex PCR technique for detection of alpha toxin gene and C.perfringens types "A" enterotoxin gene revealed that the 7 isolates of C. perfringens (100%) were positive for alpha toxin gene and only 2 out of 7 isolates (28.57%) were positive for enterotoxin gene | ||
| Keywords | ||
| Milk; C. perfringens; enterotoxigencity; Antibiotic sensitivity; PCR | ||
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