Eugenol Mitigates γ-rays-induced Acute Renal Destructive Impacts in Rats | ||
| Egyptian Journal of Radiation Sciences and Applications | ||
| Volume 36, Issue 1, 2023, Pages 1-9 PDF (1.97 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejrsa.2023.213316.1155 | ||
| Authors | ||
| Ahmed A. Elkady* 1; Rehab M. Ebrahim1; Wael A. El khouly2 | ||
| 1Health Radiation Research Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt | ||
| 2Radiation Protection Department, Nuclear and Radiation Safety Research Center (NRSRC), Egyptian Atomic Energy Authority, Cairo, Egypt | ||
| Abstract | ||
| Acute renal injury is a common and hazardous distress of γ-rays’ exposure. Aim: The aim of the current study was to explore the role of Eugenol on γ-rays-induced acute kidney lesions in rats. The rats were separated into four groups (n=6). The control group received saline for two weeks. The Eugenol group received i.p. 20 mg/kg Eugenol for two weeks. The γ-rays group was pretreated with saline for a week, then whole-body exposed to 8 Gy γ-rays and saline treatment continued for another week. The rats in γ-rays+ Eugenol group received Eugenol for a week pre-irradiation, then were exposed to γ-rays and Eugenol treatment continued for another week. The blood and kidney samples were collected for histopathological and biochemical investigations. Exposure to γ-rays induced atrophy of glomeruli with increasing capsular space, expanded renal tubules and congested inter tubular blood vessels. Histological changes were accompanied by significant decreases in the level of antioxidants: reduced glutathione content (GSH), and superoxide dismutase (SOD), and catalase (CAT) activities with a significant increase of malondialdehyde (MDA; end-product of lipid peroxidation), indicating oxidative stress. Significant increases in the levels of inflammatory markers, tumor necrosis factor-alpha (TNF-α) and interleukin-1 Beta (IL-1β) levels were recorded also. Kidney damage was substantiated by significant increases in urea, creatinine, lactate dehydrogenase (LDH), γ-glutamyltransferase (γGT), and disturbance of electrolytes balance; elevated sodium (Na+), and decreased potassium (K+). Eugenol treatment has significantly improved histological damage, oxidative stress and inflammation accompanied by amelioration of kidney functions. Eugenol treatment has significantly improved histological damage oxidative stress and inflammatory-biomarkers, which was accompanied by significant amelioration of kidney functions. Conclusion: Eugenol may alleviate acute kidney injury resulting from g-rays exposure through antioxidant and anti-inflammatory properties. | ||
| Keywords | ||
| Eugenol extract; Oxidative damage; Rats; Renal injury; γ-rays | ||
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