Determination of the genotype-adenovirus that causes multiple infections
Sahow, H., H. Abdel Aziz, S., Abdel Rahman, S., El Dougdoug,, K., A. Abo El-Maaty, S. (2023). Determination of the genotype-adenovirus that causes multiple infections. EKB Journal Management System, 8(5), 211-220. doi: 10.21608/bjas.2023.199804.1115
Hussam K.M. Sahow; Samir H. Abdel Aziz; Soheir Abdel Rahman; K. A El Dougdoug,; Sabah A. Abo El-Maaty. "Determination of the genotype-adenovirus that causes multiple infections". EKB Journal Management System, 8, 5, 2023, 211-220. doi: 10.21608/bjas.2023.199804.1115
Sahow, H., H. Abdel Aziz, S., Abdel Rahman, S., El Dougdoug,, K., A. Abo El-Maaty, S. (2023). 'Determination of the genotype-adenovirus that causes multiple infections', EKB Journal Management System, 8(5), pp. 211-220. doi: 10.21608/bjas.2023.199804.1115
Sahow, H., H. Abdel Aziz, S., Abdel Rahman, S., El Dougdoug,, K., A. Abo El-Maaty, S. Determination of the genotype-adenovirus that causes multiple infections. EKB Journal Management System, 2023; 8(5): 211-220. doi: 10.21608/bjas.2023.199804.1115

Determination of the genotype-adenovirus that causes multiple infections

Benha Journal of Applied Sciences
Article 18, Volume 8, Issue 5, May 2023, Pages 211-220    PDF (807.98 K)
Document Type: Original Research Papers
DOI: 10.21608/bjas.2023.199804.1115
Authors
Hussam K.M. Sahow* 1; Samir H. Abdel Aziz2; Soheir Abdel Rahman3; K. A El Dougdoug,4; Sabah A. Abo El-Maaty5
1Botany and Microbiology Department ,Faculty of Science, Benha University
2Professor of Molecular Genetic, Faculty of Science, Benha University
3Professor of Clinical Pathology, Faculty of Medicine, Benha University
4Professor of Virology, Faculty of Agriculture, Ain Shams University
5Assist.Professor of Virology, Faculty of Science, Benha University
Abstract
Background : Human Adenovirus (HAdV ) is considered one of the important virus that infect humans and cause multiple infections in the body,it is one of the important causes of conjunctivitis , respiratory and gastrointestinal infections, causing diarrhea. The current study was done to determine the genotype of HAdV in Egypt. Materials : The study was conducted from November 2021 to November 2022. 120 samples ( 90 stool samples and 15 conjunctival and 15 respiratory samples ) were collected from different regions of Egypt, Information related to age, sex and region were recorded . The HAdV was detected serological by ILFST test . The positive samples were cultured and HAdV isolated on Vero cell lines . HAdV was collected from infected cells and then identified using the multiplex PCR technique . The genotype HAdV was determined through bioinformatics. Results : Through this study , ILFST test , HAdV was detected in 20 (22.22%) out of 90 stool samples ,while the conjunctival and respiratory samples was negative. 12 out of 20 samples were cultured on Vero cells . The cytopathic effect CPE was observed with 6 out of 12 samples . Three samples were identified as adenovirus using the multiplex PCR , and confirmed by agarose with expected 350 bp .
Keywords
HAdV; ILFST; multiplex PCR; Vero cell lines
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