Validation of Lyophilized-Attenuated Single and Combined Canine Hepatitis, Canine Distemper, and Canine Parvovirus Vaccines
Abdel-Samad, A., Bkear, N., Khadr, A., Khodier, M., Edries, S. (2023). Validation of Lyophilized-Attenuated Single and Combined Canine Hepatitis, Canine Distemper, and Canine Parvovirus Vaccines. EKB Journal Management System, 10(1), 25-30. doi: 10.5455/djvs.2021.80072.1039
Asmaa Gamal Abdel-Samad; Nabil Mohamed Bkear; Adel Mohamed Khadr; Mohamed Hassan Khodier; Samir Mohamed Edries. "Validation of Lyophilized-Attenuated Single and Combined Canine Hepatitis, Canine Distemper, and Canine Parvovirus Vaccines". EKB Journal Management System, 10, 1, 2023, 25-30. doi: 10.5455/djvs.2021.80072.1039
Abdel-Samad, A., Bkear, N., Khadr, A., Khodier, M., Edries, S. (2023). 'Validation of Lyophilized-Attenuated Single and Combined Canine Hepatitis, Canine Distemper, and Canine Parvovirus Vaccines', EKB Journal Management System, 10(1), pp. 25-30. doi: 10.5455/djvs.2021.80072.1039
Abdel-Samad, A., Bkear, N., Khadr, A., Khodier, M., Edries, S. Validation of Lyophilized-Attenuated Single and Combined Canine Hepatitis, Canine Distemper, and Canine Parvovirus Vaccines. EKB Journal Management System, 2023; 10(1): 25-30. doi: 10.5455/djvs.2021.80072.1039

Validation of Lyophilized-Attenuated Single and Combined Canine Hepatitis, Canine Distemper, and Canine Parvovirus Vaccines

Damanhour Journal of Veterinary Sciences
Volume 10, Issue 1, July 2023, Page 25-30  XML PDF (286.17 K)
Document Type: Original Article
DOI: 10.5455/djvs.2021.80072.1039
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Authors
Asmaa Gamal Abdel-Samad email 1; Nabil Mohamed Bkear2; Adel Mohamed Khadr3; Mohamed Hassan Khodier1; Samir Mohamed Edries1
1Agriculture Research Center (ARC); Veterinary Serum and Vaccine Research Institute (VSVRI), Abasia, Cairo, Egypt
2Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt
3Faculty of Veterinary Medicine, Alexandria University, Egypt
Abstract
Through this study, we attempted to prepare and evaluate lyophilized attenuated single and trivalent vaccines of Canine Distemper (CD), Canine Adeno-1 (CA-1), and Canine Parvo (Cp type 2a) viruses. All such preparations were found to be free from foreign contaminants, safe in mice and puppies, and potent, providing vaccinated puppies with good levels of specific antibody titers against the three viruses as estimated by serum neutralization test (SNT) and indirect immune sorbent assay (ELISA). Such antibodies were found to be stable up to 12 months post-vaccination, supporting their use to protect puppies against the three virus infections with the preferable use of the trivalent vaccine, which saves effort and avoids stress factors of vaccinated puppies.
Keywords
Canine distemper; Canine Adeno-1; Canine Parvo; single vaccines; combined vaccines; Serum neutralization; ELISA
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