Enhancement of Diazinon Degradation, Isolation of Organophosphorus Hydrolase and Chemical Analysis of Metabolites | ||
| Egyptian Journal of Chemistry | ||
| Article 13, Volume 62, Issue 11, November 2019, Pages 2131-2142 PDF (1.76 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2019.11689.1748 | ||
| Authors | ||
| Nivien. A. Abosereih* 1; Ghada. M. El-sayed1; S. A. Ibrahim2; A. B. Abd El-Razik2; M.A. Hammad3; F. M. Hafez1 | ||
| 1Department of Microbial Genetics, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt. | ||
| 2Department of Genetic, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. | ||
| 3Department Plant Protections, Faculty of Agriculture, Ain Shams University, Cairo, Egypt | ||
| Abstract | ||
| In this study, diazinon high degradation was achieved by using Ethyl methane sulfonate (EMS) as a chemical mutagen, 38.19% of diazinon was degradedin the minimal media as compared to 1.83% in control media after 5 days.Due to the first step of ethylmethane sulphonate (EMS) chemical treatment, diazinon degradation increased to 62.19% which was subsequently increased to 86.21% after the EMS treatment second step. Analysis of organophosphorus hydrolaseprotein involved in diazinon hydrolysis revealed that itis a member of the MBL-fold metallo hydrolase superfamily and has beta lactamase fold.2-Iso-4-methyl-6- hydroxypyrimidine (IMHP) was detected as the main degradation product of diazinon through GC/MS analysis after 5days of incubation and further metabolized to unknown polar metabolites that weredetected using GC/MS after 10 days. | ||
| Keywords | ||
| OPs pesticides biodegradation; Diazinon; EMS; GC/MS analysis | ||
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