Prevalence and Antimicrobial Susceptibility of Ceftiofur-Resistant Enterobacteriaceae in Raw Cow’s Milk and Kareish Cheese: Implications for Public Health | ||
| Journal of Current Veterinary Research | ||
| Volume 5, Issue 2, October 2023, Pages 10-26 PDF (549.11 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jcvr.2023.320406 | ||
| Authors | ||
| Asmaa I. El Refaey; Heba Hussein; Rabee A. Ombarak; Nasser H. Abbas; Ahmed M. Hammad* | ||
| Abstract | ||
| Ceftiofur is primarily approved for the treatment of respiratory tract infections in foodproducing animals. The rise of ceftiofur resistance among Enterobacteriaceae has allegedly been attributed to the misuse and abuse of this popular antibiotic. In this study, we aimed to investigate the prevalence and antimicrobial susceptibility profiles of ceftiofur-resistant Enterobacteriaceae in raw milk and kareish cheese samples collected from Menoufia governorate, Egypt. A total of 80 samples, 40 each of raw cow’s milk and kareish cheese, were analyzed by enrichment in Enterobacteriaceae enrichment broth supplemented with ceftiofur (8 µg/ml) and plated on violet, red bile glucose agar plates with ceftiofur (8 µg/ml). The isolates were subjected to antimicrobial susceptibility testing against 10 antibiotics. The study found that 90% of raw cow's milk samples and 95% of kareish cheese samples had ceftiofur-resistant Enterobacteriaceae. Higher resistance rates to cefepime (80.95%), cefotaxime (95.24%), and ceftriaxone (95.24%) were found among raw milk isolates compared to ciprofloxacin (69.05%) and oxytetracycline (52.38%). Conversely, ceftazidime (14.29%), gentamicin (2.38%), meropenem (2.38%), and colistin (2.38%) showed lower resistance rates. Similarly, Enterobacteriaceae isolates of kareish cheese samples showed high resistance rates to cefepime (97.61%), ceftriaxone (78.57%), cefotaxime (71.43%), oxytetracycline (71.43%), and ciprofloxacin (69.05%), but low resistance rates to gentamicin (11.90%) and ceftazidime (9.52%). No resistance was observed to meropenem and colistin. Our findings suggest that raw milk and karish cheese may serve as potential reservoirs for the transmission of ceftiofur-resistant Enterobacteriaceae to humans. It is critical to monitor the frequency of ceftiofur-resistant bacteria in food items and modify the legislation controlling the use of ceftiofur in food animals. | ||
| Keywords | ||
| Antimicrobial resistance; Ceftiofur; Egypt; Enterobacteriaceae; Kareish cheese and Raw milk | ||
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