PRODUCTION AND EVALUATION OF ANTI RIFT VALLEY FEVER (RVF) IgG CONJUGATED WITH FLUORESCEIN | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 26, Volume 79, Issue 4, October 2001, Page 1621-1631 PDF (2.44 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2001.320965 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| GEHAN K. MOHAMED; MOHAMED S. WASSEL; KHIRAT A. ELIAN; FARED F. ZAKI | ||||
| Veterinary Serum and Vaccine Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt | ||||
| Abstract | ||||
| Three susceptible sheep were inoculated with RVF antigen mixed with incomplete and complete Freund's adjuvant and without adjuvant in three successive doses. The hyperimmune sera collected from the sheep were tested against standard reference RVF antigen for the presence of specific RVF antibodies using serum neutralization test (SNT), agar gel precipitation test (AGPT) and indirect fluorescent assay (IFA). Separation of RVF immunoglobulins were done using affinity chromatography (activated sepharose 46) followed by conjugation of RVF IgG with fluorescein isothiocyanale at p1-19.6. The conjugatedrRVF antibodies were titrated against reference RVF antigen and the titre reached (1:128). The working dilution was used for evaluation of different batches of attenuated and inactivated RVF vaccines in comparison with IFA test using anti-sheep Fitic, the results of both assays proved to be similar. | ||||
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