Assessment of Vascular Endothelial Growth Factor mRNA and miR-200b Levels in Type 2 Diabetes Mellites in Correlation with Clinicopathological and Anatomical Distribution of Diabetic Foot Ulcer
Rashad, N., Khalil, U., Soliman, S., Abd El-Fatah, A., Hussien, M., Elshamy, A., omar, A., Mohamed, R., salem, M., M. Nawara, A. (2024). Assessment of Vascular Endothelial Growth Factor mRNA and miR-200b Levels in Type 2 Diabetes Mellites in Correlation with Clinicopathological and Anatomical Distribution of Diabetic Foot Ulcer. EKB Journal Management System, 30(3), 767-776. doi: 10.21608/zumj.2023.240999.2937
Nearmeen Rashad; Usama Khalil; Sameh Abdel azeem Soliman; Azza Hassan Abd El-Fatah; marwa Hussien; Abdelmonem Elshamy; azza. omar; Rania Hassan Mohamed; mai salem; Abdalla M. Nawara. "Assessment of Vascular Endothelial Growth Factor mRNA and miR-200b Levels in Type 2 Diabetes Mellites in Correlation with Clinicopathological and Anatomical Distribution of Diabetic Foot Ulcer". EKB Journal Management System, 30, 3, 2024, 767-776. doi: 10.21608/zumj.2023.240999.2937
Rashad, N., Khalil, U., Soliman, S., Abd El-Fatah, A., Hussien, M., Elshamy, A., omar, A., Mohamed, R., salem, M., M. Nawara, A. (2024). 'Assessment of Vascular Endothelial Growth Factor mRNA and miR-200b Levels in Type 2 Diabetes Mellites in Correlation with Clinicopathological and Anatomical Distribution of Diabetic Foot Ulcer', EKB Journal Management System, 30(3), pp. 767-776. doi: 10.21608/zumj.2023.240999.2937
Rashad, N., Khalil, U., Soliman, S., Abd El-Fatah, A., Hussien, M., Elshamy, A., omar, A., Mohamed, R., salem, M., M. Nawara, A. Assessment of Vascular Endothelial Growth Factor mRNA and miR-200b Levels in Type 2 Diabetes Mellites in Correlation with Clinicopathological and Anatomical Distribution of Diabetic Foot Ulcer. EKB Journal Management System, 2024; 30(3): 767-776. doi: 10.21608/zumj.2023.240999.2937

Assessment of Vascular Endothelial Growth Factor mRNA and miR-200b Levels in Type 2 Diabetes Mellites in Correlation with Clinicopathological and Anatomical Distribution of Diabetic Foot Ulcer

Zagazig University Medical Journal
Article 18, Volume 30, Issue 3, May and June 2024, Page 767-776  XML PDF (790.47 K)
Document Type: Original Article
DOI: 10.21608/zumj.2023.240999.2937
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Authors
Nearmeen Rashad email orcid 1; Usama Khalil2; Sameh Abdel azeem Soliman3; Azza Hassan Abd El-Fatah4; marwa Hussien5; Abdelmonem Elshamy6; azza. omar7; Rania Hassan Mohamedorcid 8; mai salem9; Abdalla M. Nawara10
1zagazig university
2Internal Medicine, Zagazig University
3Assistant professor of Internal Medicine and Nephrology Faculty of Medicine - Zagazig University
4Lecturer of Internal Medicine, Faculty of Medicine, Zagazig University
5faculty of medicine, zagazig university
6Tropical medicine ,Zagazig university
7Medical Microbiology and Immunology
8Department of Human Anatomy and Embryology, Faculty of Medicine, Zagazig University, Egypt
9Clinical Pathology department,Research Institute of ophthalmology
10Internal Medicine Department, Faculty of Medicine, Zagazig University, Egypt
Abstract
Background: Diabetic foot ulcer (DFU) is one of the most distressing complications in patients with type 2 diabetes mellitus (T2DM). Vascular endothelial growth factor (VEGF) is a strong angiogenic factor associated with wound healing and development. Aim: This study aimed to investigate the miR-200b and VEGF mRNA levels in patients with T2DM and to discover their relations with the clinicopathological and anatomical distribution of DFU.

Methods: This case-control study enrolled 70 patients with T2DM and 70 healthy subjects as controls. Physical and neurological examination to assess the Wagner classification. RT-PCR was done to assess miR-200b and VEGF mRNA expression levels.

Results: there were significantly higher values of VEGF mRNA levels in patients with DFU (3.13±1.87) compared to patients without DFU (2.41±0.197) and controls (1.07±0.363), with P value ˂0.001* Additionally, miR-200b levels were significantly higher in patients with DFU (3.48±1.47) compared to patients without DFU (2.94±0.187) and controls (1.13±0.37), with P value ˂0.001*. The power of VEGF mRNA and miR-200b levels as a diagnostic marker for DFU were projected (2.5 and 3.1, respectively), which yielded a sensitivity of (77.1% and 74.3, respectively) and a specificity of (77.3% and 75.2%), with the AUC at (0.886 and 0.845, respectively) . The size of DFU in cm was 3.5±0.421 and the number of ulcers was 2.45±0.433.

Conclusion: the VEGF mRNA and miR-200b levels were significantly higher in patients with T2DM, particularly those with DFU. Consequently, we believed that VEGF mRNA and miR-200b may serve as promising predictive biomarkers for diabetes and DFU.
Keywords
VEGF mRNA; miR-200b; Diabetic foot ulcer; anatomical distribution; ABI
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