Assessment of Vascular Endothelial Growth Factor mRNA and miR-200b Levels in Type 2 Diabetes Mellites in Correlation with Clinicopathological and Anatomical Distribution of Diabetic Foot Ulcer | ||
| Zagazig University Medical Journal | ||
| Article 18, Volume 30, Issue 3, May and June 2024, Pages 767-776 PDF (790.47 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zumj.2023.240999.2937 | ||
| Authors | ||
| Nearmeen Rashad* 1; Usama Khalil2; Sameh Abdel azeem Soliman3; Azza Hassan Abd El-Fatah4; marwa Hussien5; Abdelmonem Elshamy6; azza. omar7; Rania Hassan Mohamed8; mai salem9; Abdalla M. Nawara10 | ||
| 1zagazig university | ||
| 2Internal Medicine, Zagazig University | ||
| 3Assistant professor of Internal Medicine and Nephrology Faculty of Medicine - Zagazig University | ||
| 4Lecturer of Internal Medicine, Faculty of Medicine, Zagazig University | ||
| 5faculty of medicine, zagazig university | ||
| 6Tropical medicine ,Zagazig university | ||
| 7Medical Microbiology and Immunology | ||
| 8Department of Human Anatomy and Embryology, Faculty of Medicine, Zagazig University, Egypt | ||
| 9Clinical Pathology department,Research Institute of ophthalmology | ||
| 10Internal Medicine Department, Faculty of Medicine, Zagazig University, Egypt | ||
| Abstract | ||
| Background: Diabetic foot ulcer (DFU) is one of the most distressing complications in patients with type 2 diabetes mellitus (T2DM). Vascular endothelial growth factor (VEGF) is a strong angiogenic factor associated with wound healing and development. Aim: This study aimed to investigate the miR-200b and VEGF mRNA levels in patients with T2DM and to discover their relations with the clinicopathological and anatomical distribution of DFU. Methods: This case-control study enrolled 70 patients with T2DM and 70 healthy subjects as controls. Physical and neurological examination to assess the Wagner classification. RT-PCR was done to assess miR-200b and VEGF mRNA expression levels. Results: there were significantly higher values of VEGF mRNA levels in patients with DFU (3.13±1.87) compared to patients without DFU (2.41±0.197) and controls (1.07±0.363), with P value ˂0.001* Additionally, miR-200b levels were significantly higher in patients with DFU (3.48±1.47) compared to patients without DFU (2.94±0.187) and controls (1.13±0.37), with P value ˂0.001*. The power of VEGF mRNA and miR-200b levels as a diagnostic marker for DFU were projected (2.5 and 3.1, respectively), which yielded a sensitivity of (77.1% and 74.3, respectively) and a specificity of (77.3% and 75.2%), with the AUC at (0.886 and 0.845, respectively) . The size of DFU in cm was 3.5±0.421 and the number of ulcers was 2.45±0.433. Conclusion: the VEGF mRNA and miR-200b levels were significantly higher in patients with T2DM, particularly those with DFU. Consequently, we believed that VEGF mRNA and miR-200b may serve as promising predictive biomarkers for diabetes and DFU. | ||
| Keywords | ||
| VEGF mRNA; miR-200b; Diabetic foot ulcer; anatomical distribution; ABI | ||
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