Assessment of Micro Ribonucleic Acid 320 Expression Level in Plasma of Vitiligo Patients Attending Suez Canal University Hospitals | ||
| Suez Canal University Medical Journal | ||
| Article 9, Volume 26, Issue 8, August 2023, Pages 0-0 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scumj.2023.322327 | ||
| Authors | ||
| Noha Z. Tawfik1; Rabab H. Abass* 1; Hoda Y. Abdallah2; Amal HA. Gomaa1 | ||
| 1Department of Dermatology and Venereology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. | ||
| 2Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. | ||
| Abstract | ||
| Background: Vitiligo is the most common skin disorder of depigmentation and is a multi-hypothesis disease requiring multifaceted interaction between autoimmune, biochemical, and genetic hypotheses. MiRNAs; are a major class of tiny noncoding RNAs. MiR-320 regulates inflammatory cytokines IL-2, IL-17, IFN-γ, and CXCL9 which are the main inflammatory cytokines contributing to the etiopathogenesis of vitiligo. Aim: To measure serum levels of miR-320 in vitiligo patients compared with controls to highlight the possible association of miR-320 with pathogenesis and severity of vitiligo. Subjects and Methods: A case-control study included 2 groups: group 1 involved 99 vitiligo patients; group 2 involved 99 healthy volunteers matched for age and sex. Disease activity and severity were assessed for each patient by VIDA and VASI scores. Serum miR-320 levels were assessed as blood samples were obtained from each patient and control than a quantitative measure of miRNA-320, using quantitative real-time PCR assay was done on all samples. Results: The mean level of miR-320 among patients was 23.05 ± 83.21; the mean level of miR-320 among controls was 1.0 ± 0.0. The variance between both groups was statistically significant (P < 0.05). Expression levels of miR-320 in vitiligo patients were higher than controls as a median of miR-320 levels in vitiligo patients was upregulated, being 3.19-fold higher than levels detected in controls. Conclusion: Serum miR-320 level was overexpressed in vitiligo patients compared to controls. Moreover, there was an insignificant association between miR-320 serum level and disease severity. Thus, miR-320 might have a key role in the etiopathogenesis of vitiligo. | ||
| Keywords | ||
| Expression; miR-320; Vitiligo | ||
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