Some studies on molecular epidemiology of Duck Hepatitis A virus in Egypt | ||
Benha Veterinary Medical Journal | ||
Volume 45, Issue 1, October 2023, Pages 74-78 PDF (1.04 M) | ||
Document Type: Original Article | ||
DOI: 10.21608/bvmj.2023.222997.1682 | ||
Authors | ||
Ahmed abdallah Mohammed* 1; Ahmed eissa Saad1; Abdelsatar arafa Mohamed2 | ||
1Department of Avian and Rabbit Diseases, Faculty of Veterinary Medicine, Benha University, Egypt. | ||
2Laboratory For Veterinary Quality Control on Poultry production, Animal Health, Dokki, Giza, Egypt. | ||
Abstract | ||
Several disease outbreaks were observed in duck farms in Egypt leading tonervous manifestations and high mortality.In this investigation,suspected Duck Viral Hepatitis samples (n=38) were collected from 38commercial flocks from five Egyptian governorates including(Sharkia, Qalyubia,Manufia,Ismalia and Dakahlia) from December 2019 to May2022 of age ranged from 3-16 days.Theinfected flocks were suffered from nervous signs with high mortality ranged from (48%-91%).There is no history of previous vaccination againstDuck Hepatitis A Virus(DHAV). Clinical samples were examined by Reverse transcription-PCRdepend on molecular identification of UTR andVP1 generespectively. The overall positiverate was 76.3% (n = 29/38). Out of 29 positive samples, five (17,24%) are subjected to sequencing and phylogenetic analysis . Our findings demonstrated that these novel DHAV strains were more similar and genetically correlated to the newly reported DHAV genotype 3 in Egypt. There were amino acid substitutions detected in VP1 gene in comparison to the first Egyptian genotype 3 (Avihepatovirus A isolate 26 VP). | ||
Keywords | ||
DHAV3; UTR; VP1; Amino acid substitutions | ||
Statistics Article View: 172 PDF Download: 235 |