PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 29, Volume 78, Issue 3, September 2000, Page 1337-1349 PDF (3.21 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2000.323909 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| SAYED M. ZEIDAN; MOHAMED M. TAHA; IMAN K. A. KASSEM | ||||
| Serum and Vaccine Production Research Institute, Agricultural Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt | ||||
| Abstract | ||||
| A preliminary study was conducted in which polycolonal antiserum against bovine viral diarrhoea virus (BVDV), was prepared in Newzeland white rabbit. The antibodies were titrated by both serum neutralization test (SNT) and enzyme linked immunosorbent assay (ELISA) techniques. The immunoglobuline was precipitated from the serum by concentrated ammonium sulphate solution and was conjugated with fluorescine isothiocyanate (FITC). Both non-reacted FITC and heavily FITC conjugated immunoglobuline were removed. The optimal dilution of the conjugate and its specificity were determined by using BVDV infected tissue cultures. Seventy-five bovine tissue specimens collected from Cairo abattoir were used for BVDV isolation, as well as for direct fluorescence antibody (FA) techniques and their results were compared. | ||||
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