Detection of GDF9 Exon2 SNPs and Investigation of their Association with Prolificacy in Two Egyptian Goat Breeds | ||
| Egyptian Journal of Veterinary Sciences | ||
| Volume 54, Issue 7, November and December 2023, Pages 199-209 PDF (1.46 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2023.236864.1618 | ||
| Authors | ||
| Aya Kotb1; Nasr E. Nasr1; Mohamed m. El-badawy2; Khaled A. Kahilo1; Mohammed Abu El-Magd* 3 | ||
| 1Department of Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt | ||
| 2Animal Production Research Institute, Agricultural Research Center, Giza, Egypt | ||
| 3Mansoura University | ||
| Abstract | ||
| The high prolific Zaraibi and low prolific Barki are among Egypt's most common indigenous goat breeds. The prolificacy of goat herds can be increased by selecting animals having single-nucleotide polymorphisms (SNPs) in fecundity-related genes. Herein, we detailed the use of DNA sequences followed by quantitative polymerase chain reaction (qPCR) and high-resolution melting curve (HRM) analysis to detect SNPs in exon 2 of the prolificacy-linked gene GDF9 and investigate their association with prolificacy in Zaraibi (n = 100) and Barki (n = 100) goats. The results of DNA sequencing (n = 20/breed) were used as a reference for genotyping the remaining DNA samples using qPCR-HRM. A non-synonymous SNP p.V371M/c.1111G>A in was detected in GDF9 of Zaraibi goats, however, Barki goats were monomorphic for this locus. The three genotypes of the c.1111G>A SNPs were significantly associated with litter size in Zaraibi goats. Homozygous mutant genotypes (c.1111AA) had significantly higher litter sizes than other genotypes (P < 0.05). Based on these findings, we recommend selecting Zaraibi goats with c.1111AA genotypes to increase the prolificacy of this breed. | ||
| Keywords | ||
| Goat; Prolificacy; GDF9; SNPs; qPCR-HRM | ||
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