In Vitro micropropagation using nodal segments from Germinated Seeds of Guava
Abd El-Khalek, A., Elaidy, A., Elfeky, A., Fetouh, M., Elkhateeb, M., Alam-Eldein, S. (2023). In Vitro micropropagation using nodal segments from Germinated Seeds of Guava. EKB Journal Management System, 2(4), 69-72. doi: 10.21608/jsaes.2023.246649.1060
Ahmed Fathy Abd El-Khalek; Ahmed Attia Elaidy; Aya Elsayed Elfeky; Mohammed Ibrahim Fetouh; Mohamed Ali Elkhateeb; Shamel Mohamed Alam-Eldein. "In Vitro micropropagation using nodal segments from Germinated Seeds of Guava". EKB Journal Management System, 2, 4, 2023, 69-72. doi: 10.21608/jsaes.2023.246649.1060
Abd El-Khalek, A., Elaidy, A., Elfeky, A., Fetouh, M., Elkhateeb, M., Alam-Eldein, S. (2023). 'In Vitro micropropagation using nodal segments from Germinated Seeds of Guava', EKB Journal Management System, 2(4), pp. 69-72. doi: 10.21608/jsaes.2023.246649.1060
Abd El-Khalek, A., Elaidy, A., Elfeky, A., Fetouh, M., Elkhateeb, M., Alam-Eldein, S. In Vitro micropropagation using nodal segments from Germinated Seeds of Guava. EKB Journal Management System, 2023; 2(4): 69-72. doi: 10.21608/jsaes.2023.246649.1060

In Vitro micropropagation using nodal segments from Germinated Seeds of Guava

Journal of Sustainable Agricultural and Environmental Sciences
Volume 2, Issue 4, November 2023, Page 69-72  XML PDF (198.94 K)
Document Type: Original research paper
DOI: 10.21608/jsaes.2023.246649.1060
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Authors
Ahmed Fathy Abd El-Khalek email orcid 1; Ahmed Attia Elaidy1; Aya Elsayed Elfeky1; Mohammed Ibrahim Fetouhorcid 1; Mohamed Ali Elkhateeb2; Shamel Mohamed Alam-Eldein1
1Horticulture Department, Faculty of Agriculture, Tanta University
2General Administration of University Hostels, Tanta University, Tanta, Egypt
Abstract
A study to micro-propagate guava (Pisidium guajava) was carried out in 2020 and 2021 at the Tissue Culture Laboratory, Horticulture Department, Faculty of Agriculture, Tanta University. Nodal segments from in vitro germinated seeds of guava were used as explants. Nodal segments (from germinated seeds) were cultured on MS media supplemented with different concentrations of benzyl adenine (BA), kinetin (KIN), or thidiazuron (TDZ) (0, 1 and 2 mg/L). 2 mg/L BA was the most favorable treatment for multiplication of shoots (21.13 shoots/explant) compared to the other treatments and the control (plant growth regulators free medium). Shoots of suitable length were transferred to the rooting media containing (0, 0.5, or 1 mg/L) IBA. The medium containing 0.5 mg/L IBA gave the highest number of roots/plantlets and the longest roots. Under greenhouse for acclimatization, the plantlets of guava derived from the medium supplemented with 0.5 or 1 mg/L indole butyric acid recorded the highest survival percentage (80%). It can be concluded that added 2 mg/L of BA in the medium gave the highest number of shoots/explant during the multiplication stage of guava shoots. In addition, added 0.5 or 1 mg/L IBA during the rooting stage increased the number of roots/plantlets with recorded a highest survival percentage after five weeks of acclimatization stage.
Keywords
Micropropagation; guava; shoot proliferation; in vitro; acclimatization
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