Isolation and Characterization of Some Haloalkaliphilic Fungi from Egypt | ||
Egyptian Journal of Chemistry | ||
Volume 67, Issue 7, July 2024, Pages 251-262 PDF (1.22 M) | ||
Document Type: Original Article | ||
DOI: 10.21608/ejchem.2023.245259.8795 | ||
Authors | ||
Noura I. Farouk; Asmaa Elhosainy; shadia Sabry; Magda A. El-Meleigy* | ||
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo Egypt. | ||
Abstract | ||
Aspergillus salinarum, Cladosporium sphaerospermum, and Penicillium camemberti were isolated as haloalkaliphiles from Egyptian soils. The three fungal isolates tolerate pH (5 – 11), and they grew on NaCl to 25% (w/v). They were properly recognized morphologically and genetically. Aspergillus salinarum is obligately halophilic fungus required at least 5% (w/v) NaCl and tolerated to 25% (w/v) NaCl at pH 10.0. They prefer KCl on NaCl, the obligate one tolerates 30%(w/v) KCl. Sucrose was preferred on sugar alcohol used followed by glycerol. Increasing glycerol concentration increased the tolerance of these fungal isolates to pH and salt. A screen for the ability to produce extracellular enzymes showed that they produce amylase, protease, cellulase and lipase on different conditions of growth. These fungal isolates have many biotechnological applications. | ||
Keywords | ||
Filamentous fungi; Haloalkaliphilic microorganisms; Extracellular enzymes- Polyextremophiles | ||
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