Isolation and characterization of actinophages specific to Streptomyces rochi from Sohag Governorate | ||||
| Journal of Sohag Agriscience (JSAS) | ||||
| Volume 8, Issue 1, June 2023, Page 136-145 PDF (928.08 K) | ||||
| Document Type: Research and Review Papers | ||||
| DOI: 10.21608/jsasj.2023.334599 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Mazhar D A Mohamed1; Christeena O F Mankarious* 1; Adel M M Hammad2; Tarek H M Elsharouny1 | ||||
| 1Agricultural Microbiology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt | ||||
| 2Agricultural Microbiology Department Faculty of Agriculture Minia University Minia Egypt | ||||
| Abstract | ||||
| The objective of this study was to identify and separate Streptomyces rochei-specific actinophages. From two different sites of reclaimed soil in the Sohag Governorate region, eight actinophage (streptomyces phage) isolates specifically for Streptomyces rochei were successfully identified. The samples were taken from reclaimed soil in Alkawmel city and the New Sohag experimental farm of the Faculty of Agriculture, Sohag University, in Sohag, Egypt. All of the isolated phages (the actinophage) looked to be head-and-tail types, according to electron micrographs of the isolated phages. The eight isolates of the phage were given the numbers 1, 2, 3, 4, 5, 6, 7, and 8. Each phage isolate's heat inactivation point was investigated. Although all phage isolates maintained their infectivity at 85 °C during 10 minutes, they all demonstrated resistance to high temperatures. It has been discovered that a pH of 7 is ideal for phage infection, while phage isolates can survive in both alkaline and acidic environments. The susceptibility of the different phage isolates to UV light varies. | ||||
| Keywords | ||||
| Actinophages; streptomyces rochi; Soahg | ||||
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