The Possible Therapeutic Effect of Adipose Derived Mesenchymal Stem Cells and N-Acetyl cysteine on Bleomycin Induced Lung Fibrosis in Adult Male Albino Rats: Histological and Immuno-histochemical Study | ||
| Benha Medical Journal | ||
| Article 2, Volume 41, Issue 6, October 2024, Pages 17-29 PDF (849.47 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bmfj.2024.225748.1867 | ||
| Authors | ||
| Wafaa Maged Metwally* 1; Omayma K. Helal2; Sherifa A.Morsy3; Nessrine E.Ahmed4 | ||
| 1Department of Histology and Cell Biology Benha Faculty of Medicine | ||
| 2Professor of Histology and Cell Biology Benha Faculty of Medicine | ||
| 3Professor of Histology and Cell Biology Benha Faculty of Medicine. | ||
| 4Ass.Professor of histology and cell biology Benha Faculty of Medicine | ||
| Abstract | ||
| Background: Pulmonary fibrosis (PF) is a chronic fibrotic lung disease. Lung parenchyma and architecture is destroyed, compliance is lost, and gas exchange is compromised in this debilitating condition that leads to respiratory failure. NAC is a precursor of L-cysteine that results in glutathione elevation biosynthesis. It acts directly as a scavenger of free radicals, especially oxygen radicals. This study aimed to evaluate the possible therapeutic effect of Adipose derived mesenchymal stem cells and N-acetylcysteine (NAC) on induced lung fibrosis in adult male albino rats. Methods: Ten rats were used to isolated adipose-derived stem cells and sixty rats were randomly divided into five groups. Group I (control group: 20 rats), Group II (affected group:10 rats), Group III (Adipose stem cell group: 10 rats), Group Iv (N-acetylcystiene group:10 rats) and Group v (Adipose stem cell + NAC group: 10 rats). Specimens taken and histological studies, immunohistochemical and morphometric study were performed. Results: Group II showed markedly thickened inter-alveolar septa and destruction with extensive cellular infiltrations, increases in macrophage and alveoli were Collapsed.And there is massive lymphocytic infiltration around bronchiolar wall.There was a significant decrease (P0.01) in Ki67 expression in group III, IV & V compared to group I. Conclusion: Each of NAC and adipose tissue-derived stem cells can protect against lung fibrosis caused by Bleomycin when administered prior to, and concomitant with bleomycin, but their combined administration have better results. | ||
| Keywords | ||
| Bleomycin; N-Acetyl cysteine; Adipose-derived Stem Cells; Picrosirius red stain; ASMA and Ki-67 | ||
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