Effect of Different Doses of Bisphenol A on Albino Rats'''' Liver: Histological and Immunohistochemical Study | ||
| Egyptian Journal of Histology | ||
| Article 4, Volume 48, Issue 1, March 2025, Pages 41-52 PDF (1.16 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2024.259809.1993 | ||
| Authors | ||
| Heba M. Fawzy* ; Eman Ali Negm | ||
| histology,faculty of medicine,ain shams university | ||
| Abstract | ||
| Introduction: Bisphenol A is a chemical-compound widely utilised as an epoxy resin in everyday products. Aim of the Work: Demonstration of the effects of different bisphenol-A dosages on the livers of adult male albino rats. Materials and Methods: Thirty adult male albino rats were split into three groups (each 10 rats). The control group is placed first, followed by low BPA and high BPA. The exposure period lasted for a total of thirty days. The measurements of serum alanine aminotransferase (ALT) and serum aspartate aminotransferase (AST) have then been performed on blood samples. Also Samples of liver tissue were collected for immunohistochemical,oxidative and histological examinations. Results: BPA administration orally at 50 and 150 mg/kg/day for 30 days caused a significant drop in glutathione peroxidase and superoxide dismutase levels. At low doses, hepatocytes had numerous bounded tiny vacuoles; at large doses, ballooning degeneration with Mallory bodies and an increase in kupffer cells and inflammatory infiltration were evident in both groups. A reduction in glycogen granules and hepatocyte apoptosis were seen in both groups, confirmed by more significant increase of Caspases 3 immunoreactivity in high dose group. Significant increases in collagen fibres in the portal tract (low dose) and persinusoidal (high dose). Vimentin expression significantly increased in both groups but more marked in the high dose group, confirming our results. Conclusion: Bisphenol oral- exposure for 30 days is linked to a number of structural and biochemical changes that point to a serious liver injury which was more marked with high dose. | ||
| Keywords | ||
| Bisphenol A; caspase- 3; liver; vimentin | ||
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