Chronic kisspeptin-10 ameliorates osteoporotic changes in orchidectomized male albino rats: involvement of Bone morphogenic protein-2 and Wnt/β-catenin signaling pathways
Habib, M., Abdelgeleel, H., Ebrahim, R. (2024). Chronic kisspeptin-10 ameliorates osteoporotic changes in orchidectomized male albino rats: involvement of Bone morphogenic protein-2 and Wnt/β-catenin signaling pathways. EKB Journal Management System, 30(8.1), 3992-4002. doi: 10.21608/zumj.2024.264538.3128
Marwa Abdelaziz Habib; Heba Abdelgeleel; Reham Ebrahim. "Chronic kisspeptin-10 ameliorates osteoporotic changes in orchidectomized male albino rats: involvement of Bone morphogenic protein-2 and Wnt/β-catenin signaling pathways". EKB Journal Management System, 30, 8.1, 2024, 3992-4002. doi: 10.21608/zumj.2024.264538.3128
Habib, M., Abdelgeleel, H., Ebrahim, R. (2024). 'Chronic kisspeptin-10 ameliorates osteoporotic changes in orchidectomized male albino rats: involvement of Bone morphogenic protein-2 and Wnt/β-catenin signaling pathways', EKB Journal Management System, 30(8.1), pp. 3992-4002. doi: 10.21608/zumj.2024.264538.3128
Habib, M., Abdelgeleel, H., Ebrahim, R. Chronic kisspeptin-10 ameliorates osteoporotic changes in orchidectomized male albino rats: involvement of Bone morphogenic protein-2 and Wnt/β-catenin signaling pathways. EKB Journal Management System, 2024; 30(8.1): 3992-4002. doi: 10.21608/zumj.2024.264538.3128

Chronic kisspeptin-10 ameliorates osteoporotic changes in orchidectomized male albino rats: involvement of Bone morphogenic protein-2 and Wnt/β-catenin signaling pathways

Zagazig University Medical Journal
Article 24, Volume 30, Issue 8.1, November 2024, Page 3992-4002  XML PDF (1.05 MB)
Document Type: Original Article
DOI: 10.21608/zumj.2024.264538.3128
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Authors
Marwa Abdelaziz Habiborcid 1; Heba Abdelgeleelorcid 2; Reham Ebrahim email 3
1Physiology department, Faculty of Medicine, Zagazig University, Egypt
2Pathology department faculty of Medicine, Zagazig University
3Physiology department faculty of medicine Zagazig university Zagazig Sharkia
Abstract
Osteoporosis is a serious worldwide skeletal illness manifested by diminished bone density with increased susceptibility to fractures and increases with aging. Kisspeptin is a hypothalamic neuropeptide that regulates the gonadal hormones and was suggested to have a positive effect on bone metabolism. Material & Methods: 30 male rats were divided equally in to three groups: Sham, orcidectomized (ORX), and kisspeptin-10 (kiss)-treated groups. Blood, urine, and femoral bone samples were taken for biochemical, histopathological, and gene studies. Results: ORX rats showed increased serum levels of alkaline phosphatase (ALP), osteocalcin, RANKL, Osteopetrogenin (OPG), RANKL/ OPG ratio, tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6(, while decreased serum OPG level and bone minerals associated with disruption of bone architecture compared with Sham group. Chronic treatment with kisspeptin-10 significantly enhanced bone architecture, increased bone minerals, improved RANKL/OPG ratio, up-regulated mRNA expression levels of OPG, bone morphogenic protein 2 (BMP-2), Wnt3a, and β-catenin as compared to untreated ORX rats. Conclusion: Chronic treatment with kisspeptin-10 enhances osteogenesis and reduces bone resorption in ORX rats via modulating RANKL/OPG ratio, BMP-2, and Wnt/β-catenin signaling pathways. So, the present study recommends kisspeptin-10 as a promising novel therapeutic agent for age-related osteoporosis in men.
Keywords
Osteoporosis; Kisspeptin; BMP-2; Wnt/β -catenin pathway
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