Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection
Salem, M., El-Naggar, S., Zidan, A., Elbatei, H., Hamed, E., Hamada, H. (2017). Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection. EKB Journal Management System, 1(1), 23-30. doi: 10.21608/jcbr.2017.34736
Mohamed Salem; Sabry A El-Naggar; Abdel-Aziz A Zidan; Hasan Elbatei; Ezar H Hamed; Hossam Y. Hamada. "Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection". EKB Journal Management System, 1, 1, 2017, 23-30. doi: 10.21608/jcbr.2017.34736
Salem, M., El-Naggar, S., Zidan, A., Elbatei, H., Hamed, E., Hamada, H. (2017). 'Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection', EKB Journal Management System, 1(1), pp. 23-30. doi: 10.21608/jcbr.2017.34736
Salem, M., El-Naggar, S., Zidan, A., Elbatei, H., Hamed, E., Hamada, H. Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection. EKB Journal Management System, 2017; 1(1): 23-30. doi: 10.21608/jcbr.2017.34736

Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection

Egyptian Journal of Cancer and Biomedical Research
Article 5, Volume 1, Issue 1 - Serial Number 5, October 2017, Pages 23-30    PDF (1.9 M)
Document Type: Original Article
DOI: 10.21608/jcbr.2017.34736
Authors
Mohamed Salem* 1; Sabry A El-Naggar1; Abdel-Aziz A Zidan2; Hasan Elbatei3; Ezar H Hamed1; Hossam Y. Hamada1
1Department of Zoology, Faculty of Science, Tanta University, Egypt
2Department of Zoology, Faculty of Science, Damanhur University, Egypt
3Faculty of Medicine, Kafr Elsheikh University, Egypt
Abstract
Introduction: Infected patients with chronic hepatitis C virus (HCV) showed high numbers of myeloid-derived suppressor cells (MDSCs) which are known to suppress the immune response. Given that microRNAs regulate the expression of many genes, it is important to test whether MDSCs correlate with altered miRNA expression. Aim: The present study aimed to assess the expression of miR-29, 122 and 155 in chronic HCV patients under interferon (IFN) therapy and their correlation with the frequency of MDCS. A total of 28 subjects, including eighteen chronic HCV patients, and 10 healthy volunteers were enrolled in this study. MiR-29, 122 and 155 were evaluated using real-time- polymerase chain reaction (RT-PCR). Flow cytometric analysis was performed to evaluate the MDSCs which defined as Lin-HLA-DR- CD33+ CD11b+. Results: miR-29 and 122 expressions in HCV patients showed significant decrease when compared to their control. However, there was an increase (but non-significance) in miR-155 in HCV patients than control subjects. Both relative and absolute numbers of MDSCs were increased in the peripheral blood in HCV patients as compared to control subjects. Moreover, non- significance change in IFNα/β receptors was observed in HCV patients as compared to control subjects. Conclusion: High numbers of MDSCs in chronic HCV patients correlate with low expression of miR-29 and122
Keywords
Micro RNA; Gene expression; Egyptian patients; HCV; MDSCs
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