Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections | ||
| Journal of Bioscience and Applied Research | ||
| Volume 10, Issue 1, March 2024, Pages 85-102 PDF (1.45 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jbaar.2024.348053 | ||
| Authors | ||
| Noor Ahmad* 1; Qudus Wamidh2; Thanaa Rasheed Abdulrahman2 | ||
| 1Department Microbiology, Medical College/Al Nahrain University, Iraq | ||
| 2Department Microbiology Medical College/Al Nahrain University, Iraq | ||
| Abstract | ||
| Background: Urinary tract infections (UTIs) frequently affect the urinary system, predominantly caused by Escherichia coli (E. coli) and Klebsiella pneumoniae. This study examines the prevalence of these bacteria and bla OXA-48 and bla IMP genes. Objectives: The research aims to assess the occurrence of MDR and XDR E. coli and K. pneumoniae in pediatric UTI cases, including the resistance genes bla OXA-48 and bla IMP. Methods: A total of 100 pediatric UTI urine samples were analyzed for antibiotic resistance, ESBL detection, and bla OXA-48 and bla IMP gene presence using molecular techniques, gene sequencing, and statistical analysis via SPSS software. Results: High resistance rates to common antibiotics were found in K. pneumoniae and E. coli isolates. bla OXA-48 was detected in 92.3% of E. coli and 100% of K. pneumoniae isolates, while bla IMP was found in 43.1% of E. coli and 25.7% of K. pneumoniae isolates. No nucleic acid variation was observed in the blaIMP-1 sequences of both bacteria. Conclusion: The significant presence of bla OXA-48 and bla IMP genes in K. pneumoniae and E. coli highlights the urgent need for enhanced surveillance and careful antibiotic use to curb antibiotic-resistant UTIs in children.Bottom of Form | ||
| Keywords | ||
| bla OXA-48 gene; bla IMP gene; Urinary tract infections; E. coli; Klebsiella pneumoniae | ||
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