The Therapeutic Potential of Platelet- Rich Plasma and/or Mesenchymal Stem Cells on Diabetic Wound Healing: Histological and Immunohistochemical Study | ||||
| Egyptian Journal of Histology | ||||
| Article 11, Volume 48, Issue 2, June 2025, Page 540-572 PDF (3.93 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2024.276171.2040 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Badria Hassan Saad  1; Eman Elazab Beheiry 2; Nancy Mohamed El Sekily3; Dina Mohamed Rostom4; Samar S Ibrahim5; Rasha R. Salem 6
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| 1Human anatomy and embryology department, Faculty of medicine, Alexandria university, Alexandria, Egypt | ||||
| 2anatomy and embryology department, faculty of medicine, alexandria university | ||||
| 3Human Anatomy and Embyrology Department, Faculty of Medicine, Alexandria University, Egypt | ||||
| 42Histology and cell biology department, Faculty of medicine, Alexandria University, Alexandria, Egypt | ||||
| 5Permanent researcher Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA). Faculty of Medicine, Alexandria University. | ||||
| 6Anatomy department,faculty of medicine.alexandria university | ||||
| Abstract | ||||
| Introduction: Non-healing wounds are a significant public health issue and a significant financial drain on the healthcare system. Platelet-rich plasma (PRP) was proved to be successful in numerous experiments as it is rich in many cytokines and proteins important for wound healing. Bone marrow- derived mesenchymal stem cells (BM-MSCs) release trophic factors that control inflammation and remodelling, thus it can aid in wound healing. Objective: The aim of this study was to assess morphological, histological and immunohistochemical effects of PRP and/or BM-MSCs on cutaneous wound healing in diabetic rats. Materials and Methods: Hundred healthy male albino rats; ten rats were used as mesenchymal stem cells and PRP donors. Eighteen rats were used as negative control group (A). Seventy-two rats (B) were divided into 4 groups 18 rats each after induction of diabetes by streptozotocin (STZ) drug and formation of skin wounds; Group I: positive control group, Group II: received single dose of PRP injection in the wound, Group III: received single dose of BM-MSCs injection in the wound, Group IV: BM-MSCs plus PRP. Cutaneous wounds were inspected and photographed every 2 days of the study period for morphological study. Six rats from each group were operated upon on days 7, 14, 30 of the study for histological and immunohistochemical studies. Results: The best healing results were seen in group IV regarding morphological, histological and immunohistochemial results with a statistically significant difference between group IV and other study groups. Group III showed better healing results than in group II which was better than that of group I. Conclusion: There is a synergistic effect of combined BM-MSCs and PRP on healing of skin wounds in STZ-induced diabetic rats which is better than to use either of them alone | ||||
| Keywords | ||||
| Bone marrow derived mesenchymal stem cells; cutaneous wounds; diabetes; platelets rich plasma | ||||
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