Characterization of plasmid mediated quinolone resistance genes in Enterobacteriaceae in a University Hospital in Tunisia | ||
| Microbes and Infectious Diseases | ||
| Article 19, Volume 5, Issue 4, November 2024, Pages 1431-1437 PDF (633.75 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2024.279518.1865 | ||
| Authors | ||
| Sana Bahri1, 2; Sabrine Jarbouii3, 4; Ikram El Ahmer4, 5; Aniss Bel Haj Khalifa4, 5; Mohamed Khedher4, 5; Mahjoub Aouni3, 4; Olfa Bouallegue2, 6; Wejdene Mansour* 1, 2 | ||
| 1Laboratoire de recherche « Biophysique métabolique et Pharmacologie Appliquée » LR12ES02, Tunisie | ||
| 2Faculté de médecine de Sousse, Université de Sousse, Tunisie | ||
| 3Laboratoire de recherche « Maladies transmissibles et substances biologiquement actives » LR99ES27, Tunisie | ||
| 4Faculté de Pharmacie de Monastir, Université de Monastir, Tunisie | ||
| 5Laboratoire de Microbiologie, Hopital Tahar Sfar, Mahdia, Tunisie | ||
| 6LR20SP06, laboratoire de Microbiologie, Hôpital Universitaire Sahloul, Sousse –Tunisie | ||
| Abstract | ||
| Background: Quinolone-resistant enterobacterial isolates have spread largely across hospitals and community in last years. In Tunisia, numerous studies describe the emergence of plasmid mediated quinolone resistance (PMQR) genes among Enterobacteriaceae. Objectives: Detection of PMQR genes among a collection of enetrobacterial isolates recovered in the Tahar Sfar University hospital in Tunisia and in the community. Methods: In vitro antimicrobial susceptibility testing, extended-spectrum β-lactamases, and PMQR genes were detected using PCR. Clonality of isolates was assessed by ERIC-PCR. Results: In this work, 1710 enterobacterial isolates were recovered in the Tahar Sfar University Hospital in Tunisia between January 2012 and March 2013. Eighty of them were resistant to nalidixic acid: 61 isolates and 19 isolates were isolated from nosocomial and community cases respectively. Detection of PMQR genes leads to the identification of 7 and 8 QnrB producers in nosocomial and community acquired strains respectively. The qnrS PMQR gene was less present with 5/61 cases in nosocomial starins and 1/19 case in community one. The most predominant ESBL was the CTX-M-type one. According to ERIC-PCR profiles, we note a multiclonal dissemination with 12 different profiles in hospital-acquired strains and 9 profiles in community enterobacterial isolates. Conclusion: This result re-emphasize the widely distribution of the QNRB genes and their role in resistance to fluoroquinolones. | ||
| Keywords | ||
| qnr; quinolone resistance; Enterobacteriaceae; Tunisia | ||
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